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11 29 Spotted CDNA Sample Form London Regional
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cDNA microarray and oligonucleotide array
cDNA microarray steps
Microarray vs whole genome sequencing
Oligonucleotide microarray steps
DNA microarray types
DNA microarray PDF
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People also ask
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What is the purpose of cDNA microarray?
The cDNA microarray is the most powerful tool for studying gene expression in many different organisms. It has been successfully applied to the simultaneous expression of many thousands of genes and to large-scale gene discovery, as well as polymorphism screening and mapping of genomic DNA clones.
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What is microarray and how does it work?
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips.
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How do microarrays measure gene expression?
Following hybridization, the microarray is scanned to measure the expression of each gene printed on the slide. If the expression of a particular gene is higher in the experimental sample than in the reference sample, then the corresponding spot on the microarray appears red.
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What advantage do oligonucleotide arrays have over cDNA arrays?
Oligonucleotide-based microarrays offer a number of advantages over cDNA microarrays, including (i) more controlled specificity of hybridization, which makes them particularly useful for the analysis of single nucleotide polymorphisms (17) or mutational analysis (18,19); (ii) versatility to address subtle questions ...
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How are microarrays used to measure gene expression?
Following hybridization, the microarray is scanned to measure the expression of each gene printed on the slide. If the expression of a particular gene is higher in the experimental sample than in the reference sample, then the corresponding spot on the microarray appears red.
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How is gene expression measured?
Gene expression measurement is usually achieved by quantifying levels of the gene product, which is often a protein. Two common techniques used for protein quantification include Western blotting and enzyme-linked immunosorbent assay or ELISA.
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How microarrays are used in the analysis of genomes?
When a microarray is used to analyse genomes: DNA is collected from the species going to be compared. Restriction enzymes are used to cut the DNA into fragments. These fragments are denatured to create single-stranded DNA molecules.
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What are microarrays used for?
Microarray technology can be used for a variety of purposes in research and clinical studies, such as measuring gene expression and detecting specific DNA sequences (e.g., single-nucleotide polymorphisms, or SNPs ).
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