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Design and Application of New 16s Rrna Targeted Oligonucleotide Probes for the Azospirillum Skermanella Rhodocista Cluster Form

Design and Application of New 16s Rrna Targeted Oligonucleotide Probes for the Azospirillum Skermanella Rhodocista Cluster Form

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Video instructions and help with filling out and completing Design And Application Of New 16s Rrna Targeted Oligonucleotide Probes For The Azospirillum Skermanella Rhodocista Cluster Form

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Instructions and help about oligonucleotide probes

today's talk is about uh how to design primers and real-time probes um they are called a number of things um they are technically oligonucleotides which means um many nucleotides but for short we just call them oligos so i might call them oligos i probably will not call them oligonucleotides um [Music] okay here we go so first of all i'm going to talk about some things that you need to think about before you even start designing your primers and or probes um we're going to talk about the characteristics of the template what you need to know about your template and why i will go over primer design rules then i'm going to talk to you about primer synthesis which might seem a little off the beaten path but i think that if you understand it you have a much better opportunity to troubleshoot it if that comes up and then i'm going to talk about real-time probes and the designing for the primers and probe sets [Music] okay so the nature of the template

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