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Cp58 Sample Form

Cp58 Sample Form

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[Music] I'm Doug with Life Technologies and in this segment of as Tac man we're covering questions about assay design it's a common concern for people who have done a lot of gel-based PCR over the years and designed hundreds of primer sets but never for real-time PCR you might be asking yourself do I need to do anything special first off size definitely matters when it comes to amplicons in realtime PCR be sure not to make your products too large as this can contribute to a less than optimal PCR efficiency Instead try to keep them somewhere between 50 and 150 bases long second and this is critical for quantitative experiments don't design oligos to a mismatch or to a known polymorphic site is this might cause inefficient binding during the first few PCR cycles and shifts CTS to the right instead always confirm that you have the correct sequence third be careful about designing to sequences that aren't unique be especially mindful of homologues wh

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