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when the canister package arrives be sure the custody seal has not been broken open the packaging and remove the equipment chain of custody form to15 sample information form and support documents if sampling with multiple canisters match the can with the regulator number on the chain of custody form remove the brass dust cap on the canister attach the regulator to the six liter can using your fingers being careful not to cross thread the nut tighten until snug with a 9/16 inch wrench only after finger threading make sure the brass dust cap on the regulator has been removed if present in let it dangle open the valve by turning it counterclockwise until the valve is completely open sampling will now begin record the initial vacuum on the chain of custody it should read between minus 25 and minus 30 inches of mercury if not close the valve and check the threading to see if it is tight on the cam after the sampling time has elapsed stop sampling by closing the valve on the canister by turn

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