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QUADAS 2 Evaluation Form National Center for Biotechnology Ncbi Nlm Nih

QUADAS 2 Evaluation Form National Center for Biotechnology Ncbi Nlm Nih

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Instructions and help about quadas2 pdf

in this video we're going to show you how to do a laboratory develop test an ldt on the co flu and RSV qpcr assay you can use this as a template for any molecular assay the first thing you have to do is you have to make a validation plan the validation plan needs to be signed off by your medical director before you begin a great place to store your validation plan is media lab that's great for document tracking so when you're doing a validation plan what you want to include is sensitivity study basically your lods how low can you go the second one you want to do is specificity which is making sure you're hitting the right targets you also want to do an accuracy study is positive positive is negative negative and the last thing you want to do is a Precision study that's your replicates right whether it's 20 replicates or 30 replicates you want to make sure that the kit is extremely robust since this is a qualitative assay you're no

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