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Astm F1980 16 PDF  Form

Astm F1980 16 PDF Form

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this is a picture of EBS that we made in he ate and cells that were cultured in yate and on vitronectin and then moved to e8 in suspension cultures I was not sure because it only has eight components whether eard alone would be able to make eb's and so we had experimental conditions where we had just eh we had eh let's Quinn's SPS Quinn serum protein substitute is similar to human serum albumin it's used a lot in IVF clinic and we had access to some of it so we chose to use it you can use human serum albumin you can use fetal bovine serum for that matter we just wanted to keep everything zeno free and then our control was teaser + quince SPS the only caveat to this is you have to have low fgf so typically we use hundred nanograms when we have when we make eb's with our culture medium we use 40 nano grams of fgf and so as you can see all of the conditions made embryo and bodies and we looked at them at day 3 day nine day 14 and a 22 and we stop th

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