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there are several enzymes that modify the DNA molecules by addition or removal of specific chemical groups those are known as DNA modifying enzymes let's know about few DNA modifying enzymes one by one first example of DNA modifying enzyme is alkaline phosphates which is obtained from eoli or car intestinal tissue in gen cloning process this enzyme is used to remove phosphate group groups from the five Prime end of DNA molecules as you know at the five Prime end phosphate group is present in DNA molecule which is replaced by o group at the five Prime end of that DNA molecule by the action of alkaline phosphides this is also used as a reporter Gene for identification of secretion signals second example is polynucleotide kyes which is obtained from eoli infected with T4 F virus now in this case it has a reverse effect to alkaline phosphates that means it transfers phosphate of ATP to a five Prime o and in DNA or RNA in this figure you can see that five Prime o uh of DNA is conver

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