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Bacterial Transformation Worksheet

Bacterial Transformation Worksheet

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hi everyone doc romba are here to explain some of the principles involved in bio-rad's pee glow bacterial transformation lab as well as to review the basic protocol and what to expect when you're doing this lab so essentially what we're doing is taking a genetically engineered plasmid and transforming bacteria with it through a heat shock protocol we can then use the you know the principles of the scientific method use our scientific reasoning to show how a bacterium can take up a plasmid and express the genes in that plasmid and exhibit the traits created by the proteins generated so it's really an important fun lab to reinforce or protect perhaps teach gene expression to your students so basically what we're seeing here is the act of transformation taking a plasmid putting it into a subset of bacteria growing up that bacteria and then making those bacteria Express the gene make a protein eventually show this trait and in this case it's

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