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ed fo Tech instructional videos transformation for this experiment you will need an e coli source plate a tube of plasmid DNA nutrient media plates and recovery broth ice-cold calcium chloride solution and an ice bucket a micropipette toothpicks transfer pipettes and sterile loops microcentrifuge tubes two water baths set to 37 degrees Celsius and 42 degrees Celsius and finally an incubator set to 37 degrees Celsius step 1 label 1 micro centrifuge tube plus DNA and a second microcentrifuge tube - DNA step 2 transfer 500 microliters of ice-cold calcium chloride solution into the - DNA tube using a sterile 1 mil pipette step 3 using a toothpick transfer 8 to 10 well isolated colonies from the e.coli source plate to the - DNA - step 4 vigorously twist the toothpick between your fingers to free the cells step 5 fully resuspend the bacterial cells in the calcium chloride solution until no clumps of cells are visible the cell suspension should look howdy step 6 transfer 250 microliters of th

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