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Iso 6492 Form

Iso 6492 Form

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hello my name is andre chabelski and i'm from saint petersburg state university the title of my talk is isaac 1 as a form analysis and quantification with long error-prone transcriptomic reads i will talk about our tool that we designed for long read rna analysis the pipeline is pretty simple we take as input agent annotation and long read alignments that you can obtain with your favorite spliced aligner for example minimap gmap or star law then we match align reads against known transcripts we try to assign each read to a known either form detect inconsistencies between mapped reads and known isoforms and count different features such as genes transcripts and exons further we split reads into two different categories consistent splice meshes which most likely represent known transcripts and inconsistent splice matches which most likely correspond to novel transcripts or events such as intron retention we further group these reads by the inconsistency type and attempt to create

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