Padlock Oligonucleotides for Duplex DNA Based on Sequence Pnas Form

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at high temperatures double-stranded DNA will denature or separate into single strands when the temperature is lowered the two strands will anneal because of the base pairing interactions of the complementary strands if the nucleotide sequences are similar complementary strands from different sources will also anneal this is called hybridization to indicate that each strand of DNA came from a different source regions of double-stranded DNA in which the respective single strands hybridize to one another are said to be homologous or have similar or identical nucleotide sequences the high specificity of base-pairing interactions between complementary strands of DNA can be used to locate a specific nucleotide sequence in a sample if the DNA from one source is immobilized by attachment to a solid surface such as nitrocellulose homologous DNA from another source will hybridize and be retained by the immobilized DNA this is the basis for various DNA probe techniques non-homologous DNA will no