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Sequence AlignmentMap SAM Format Cornell University Chagall Med Cornell

Sequence AlignmentMap SAM Format Cornell University Chagall Med Cornell

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this is a production of Cornell University awesome cool okay well today I'm going to be sharing the work of a whole host of people uh shown here on the screen but first I would like to introduce us to the maze classical Gene iodine Japonica one now this chain causes the leaf variegation striping that you see here it was cloned in 1992 and it's shown here having five exons now if I want to study this Gene today I would go to a remains reference genome browser where I would promptly see that our V5 annotation version only has three exons when I was expecting to see five so the question is what causes this discrepancy and while the answer is that Gene model annotation is really really hard and this is due both to computational errors and biological noise so if we start by thinking of just the gene models that we know are being translated into proteins we're going to run into problems right off the bat from alternative splicing if we expand out and we start looking

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