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Cloned Human Neuropeptide Y Receptor Couples to Two Different  Form

Cloned Human Neuropeptide Y Receptor Couples to Two Different Form

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(upbeat music) - [Instructor] Welcome to this video on how to simulate In-Fusion cloning with Geneious Prime. In-Fusion cloning allows the combination of multiple DNA fragments with homologous ends. The In-Fusion enzyme chews back the nucleotides from the three prime ends of the fragments. This creates overlapping homologous ends on the different fragments which can then anneal together. Addition of DNA polymerase fills in any non-complementary sequences, and finally, DNA ligase seals adjacent nicks. The result of In-Fusion cloning is a scarless joint of multiple DNA sequences. That's a brief overview of In-Fusion cloning. Let's now simulate this process in Geneious. In this example, we will clone a promoter sequence into a vector and have this placed before the EGFP gene. The first step is to prepare your DNA fragments for cloning, such as a vector and an insert. Vectors used in In-Fusion cloning need to be linearized first. To prepare a vector for In-Fusion cloning, i

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