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ATTACHMENT 1 BAL 07 403 Opm  Form

ATTACHMENT 1 BAL 07 403 Opm Form

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we're going to be talking about massively parallel sequencing which you may also hear referred to as next-generation sequencing the objectives of this lecture are to become familiar with the general theory of how massively parallel sequencing works and then also to become aware of some of the pre analytical analytical and post analytical metrics that you want to pay attention to to help guide the interpretation of results from these platforms to begin with we should talk about the difference between traditional Sanger sequencing versus next generation or massively parallel sequencing Sanger sequencing starts with the amplification of a single amplicon of interest and the millions of copies that are generated through polymerase chain reaction are all sequenced at the same time in that setting the Sanger sequencing is highly precise however the molecule that is most abundant in the mixture that you made during your polymerase chain reaction is the molecule that you'll be

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