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Agarose Type I Easternct Form
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People also ask
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Is agarose a synthetic?
Agarose is a polysaccharide that is isolated and purified from agar or agar-bearing marine algae (sea kelp). It is a natural polymer, made up of alternating β-D-galactose and 3,6-anhydro-L-galactose units of agarobiose in its chemical structure.
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What agarose is best for electrophoresis?
1.5% agarose gel is right option if you want to check pcr product. To resolve DNA fragments between 50 - 500 base pair, its better to use PAGE.
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Why is 2% agarose gel used?
Higher percentage gels are better for separating smaller DNA fragments. The photos below compare the same DNA fragments run for the same amount of time on a 2% gel vs. a 1% gel. Notice that separation of the smaller fragments (200-50 bp) is much better on the 2% gel.
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Why use a 3% agarose gel rather than a 1% agarose gel?
Increasing the agarose concentration of a gel reduces the migration speed and enables separation of smaller DNA molecules.
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What is the best agarose for gel electrophoresis?
1.5% agarose gel is right option if you want to check pcr product. To resolve DNA fragments between 50 - 500 base pair, its better to use PAGE.
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Why do we use 1.5% agarose gel?
For separating DNA fragments between 600 bp and 5 kb we use 1% agarose gels. Higher resolution in the 300 bp - 1 kb range can be achieved using 1.5% agarose.
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What is low EEO agarose?
Low EEO agarose is a high quality molecular biology grade agarose suitable for analytical and preparative electrophoresis of nucleic acids used in the separation of high molecular weight nucleic acids in low gel concentrations. Specification: Moisture. ≤ 10% Gel strength (1.5% gel)
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What are the 2 types of gel agarose?
Agarose LE stands for low electroendosmosis, making it well suited for PCR analysis and preparative electrophoresis. General purpose agarose, on the other hand, is used for routine electrophoresis. For quick clarification, preparative electrophoresis refers to the purification of nucleic acids from an agarose gel.
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