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11 26 Affymetrix ChIP Sample Form London Regional

11 26 Affymetrix ChIP Sample Form London Regional

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so fraser gave a really nice talk about what you can get out of ffpe and also he touched upon at the very end the technical problems that ffp samples really present us with and as a scientist by training so i'm not a pathologist i'm not a medic i'm innately skeptical and when i embarked on the project that i'm going to tell you about i was skeptical from the outset so i wanted to truly test the technology to see whether or not we could actually get anything meaningful out of ffpe samples so we've heard before that ffpe is degraded this is a really nice biome analyzer plot here showing undegraded cell line rna with two ribosomal peaks this is a representative cervix sample that's been stored for about 10 years and what you can see is instead of these peaks you end up with a bump of what is essentially degraded cross-linked mutated rna we know that dna is a lot easier to work with and protein even more easy but ffpe rna is probably the wors

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