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when determining the half-life of the drug we must first figure out how many times you have to cut that amount in half so let's look at a drug level that started out at 16 milligrams per deciliter but ended at 2 milligrams per deciliter as you can see we have 16 divided by 2 which is 8 8 divided by 2 becomes 4 4 divided by 2 becomes 2 the original amount was cut in half 3 times within that time frame now if we look at the elapsed time let's say the elapsed time was 6 hours so 2 milligrams per deciliter was read at 6 hours after the 16 milligrams per deciliter we would need to take that 6 hours and divide by 3 because we had 3 times that we could cut it in half the result would be 2 hours that would be your half-life this is the calculation we use to figure out the concentration of an analyte in a patient sample using a spectrophotometer the calculation says that we need to take the absorbance of an unknown divided by the absorbance of your standard times the concentrati

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