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Ospra 102 Form

Ospra 102 Form

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Instructions and help about Ospra 102 Form

welcome to the ask a scientist video series in this episode we'll talk about fold 80 base penalty a measurement of sequence uniformity and targeted ngs there are two important closely related metrics to consider when examining sequencing data following target enrichment specificity and uniformity specificity is the percent of reads on target and optimizing for specificity ensures that sequencing reads are not wasted on off target regions however that's not enough you also need to ensure that the on target reads are aligned and evenly across all targets that's uniformity the fold 80 base penalty is one metric used to measure uniformity fold 80 penalty is the amount of additional sequencing that's required to ensure eighty percent of the target bases achieve the original mean coverage when ideal uniformity is achieved shown on the left then the on target rate is a hundred percent with no off target reads and uniformly distributed reads across all of the ta

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