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XF96 Assay Preparation Checklist Seahorse Bioscience Form

XF96 Assay Preparation Checklist Seahorse Bioscience Form

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a 2009 publication in molecular cancer therapeutics further validates Howie Carr correlates with the lactic acid produced fire glycolysis in this study z8l generated an a5 for nine knock down four fumarate hydratase creating a Krebs cycle deficient line and in a five for nine knocked out for both fh and lactate dehydrogenase a creating a glycolysis deficient line lactate accumulation in e car within measured in a color metric assay and using the EXIF analyzer respectively as expected the fh knockdown cells displayed a compensatory increased lactate formation as measured by lactate accumulation and real-time measurements in annex f24 analyzer compared with control cells while the fh LDH a's deficient cells displayed reduced lactate production in summary using a seahorse xf analyzer we are able to demonstrate unique phenotypes of cell lines by simultaneously measuring respiration and glycolysis rates in response to bio energetic pathways perturbations

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