XF96 Assay Preparation Checklist Seahorse Bioscience Form
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People also ask
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What is the seahorse glycolysis assay?
The Agilent Seahorse Glycolysis Stress Test kit measures the capacity of the glycolytic pathway after glucose starvation. This is accomplished by driving cells toward glycolysis and assessing their ability to increase glycolytic activity to meet metabolic and bioenergetic demands. -
How do you set up a seahorse assay?
Open the Agilent Seahorse Flux Assay Kit and remove the contents. Place the Sensor Cartridge upside down next to the Utility Plate. Fill each well of the Utility Plate with 1 mL of XF Calibrant. Place the Hydro Booster on top of the Utility Plate. -
What is seahorse XF used for?
The Seahorse XF-96 measures the rate of change of analytes dissolved oxygen and pH in media immediately surrounding living cells cultured in a microplate. Changes in the extracellular media are caused by the consumption or production of analytes by the cells. -
How long does it take to run a seahorse assay?
Total assay time is typically 60 to 90 minutes. Upon completion of an XF assay, other types of biological assays such as cell viability can be performed on the same plate. To maintain normal cell physiology, a temperature control system maintains the cellular environment at 37°C (or other pre-set temperature). -
What is the seahorse assay for mitochondrial function?
The Seahorse XF Cell Mito Stress Test is the gold standard assay and is widely used for measuring mitochondrial function in cells. This assay provides insight into the cause of mitochondrial dysfunction and an in-depth understanding of metabolic pathways, signals, and phenotypes. -
What is seahorse XFe96 analysis?
Agilent Seahorse XFe96 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. -
What is the seahorse XF assay?
The Agilent Seahorse XF ATP Real-Time rate assay measures and quantifies the rate of ATP production from glycolytic and mitochondrial system simultaneously using label-free technology in live cells. In mammalian cells, glycolysis and oxidative phosphorylation (OXPHOS) pathways provide the majority of cellular ATP. -
What does seahorse XF measure?
Seahorse XF technology measures the flux of oxygen, the oxygen consumption rate [OCR], and the flux of protons, the extracellular acidification rate [ECAR], in the medium immediately surrounding cells in a microplate. OCR is proportional to mitochondrial respiration, while ECAR is proportional to glycolysis.
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