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[Music] misfolding of tau protein and its self-assembly into paired helical filaments is a pathological hallmark of alzheimer's disease and other thyopatis here we present a robust tau aggregation assay that could help elucidate the tau pathogenesis process the conversion follows all the steps of a nucleation dependent polymerization process and is highly reproducible allowing drug screening in a more rigorous format that methods available to date the quality of reagents is crucial the recombinant town needs to be highly pure and devoid of aggregates and or fragments first turn on the computer and the multi-mode microplate reader after allowing the equipment to stabilize start the software and select standard protocol as the protocol type set the temperature to 37 degrees celsius and select preheatment before continuing the protocol next set the kinetic run time to 50 hours and the measurement interval to 15 minutes set the orbital shaking to 425 cycles per minute in continuous

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