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Ct 3 Fillable Form

Ct 3 Fillable Form

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in this video I'm going to show you how to perform the delta delta CT method for calculating gene expression values from a real-time PCR experiment by using Microsoft Excel so I've got some practice data here so let's say I've performed a PCR experiment from a series of control and treated samples so I've got three replicates three biological replicates in each group so I've got control one two and three and treated one two and three and upon my PCR experiment for a housekeeping gene which is here and my gene of interest which is here and within that I did duplicate wells so I've got two CT values for each sample so the first thing you should do if you've got individual CT values for each sample is to average these values so I'm going to go ahead and just create a new header so we're going to call this average CT so I'll do this for the housekeeping gene so obviously in Excel it's the formul

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