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Multiplex Analysis of Cytokines in Exhaled Breath Condensate Form

Multiplex Analysis of Cytokines in Exhaled Breath Condensate Form

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before collection the aluminum cooling sleeve is stored in a normal household freezer the cold sleeve is then inserted into our insulating cover and placed over the are to the walls of the collection chamber are now ice-cold and ready for collecting exhaled breath condensate the subject breeze calmly through her mouth inhaling warm air and exhaling through the cold our tube which efficiently condenses her excelled breath during inhalation ambient air travels through a one-way valve and into the lungs exhale there is then channeled through a second one-way valve and through the cold collection chamber where aerosols and certain volatile compounds are trapped during condensation after breathing for a predetermined period of time caps are placed over the ends of the our tube her name and date can be recorded on the label and the our tube is stored in the freezer once the required number of samples have been collected the our tubes are easily transported to the laboratory using a small ice

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