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People also ask
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What is a cell type specific gene?
The CTS genes are defined as the genes with strong differential expression among cell types. These genes are often of great interest because they are closely related to the cellular identity and function, and potentially the pathologies of different diseases [9,10,11].
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Which is the most popular method of gene silencing?
Currently, siRNAs are being widely used to suppress specific gene expression and to assess the function of genes.
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What is the mechanism of virus induced gene silencing?
Virus-induced gene silencing (VIGS) silences a gene of interest (GOI) by exploiting plant defense mechanisms against RNA viruses. A GOI fragment is cloned into a construct containing viral genome sequence (1) and then inoculated into plants (2), where viral particles are produced (3).
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What is the mechanism of siRNA gene silencing?
The siRNA-induced post transcriptional gene silencing is initiated by the assembly of the RNA-induced silencing complex (RISC). The complex silences certain gene expression by cleaving the mRNA molecules coding the target genes.
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How do siRNA molecules silence genes?
Small double-strand siRNAs are transfected into cells where the guide strand is loaded into RISC. This activated protein and nucleic acid complex can then elicit gene silencing by binding, through perfect complementarity, to a single target mRNA sequence, thereby targeting it for cleavage and degradation.
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How does siRNA treat gene silencing?
Small interfering RNA (siRNA) is a potential method of gene silencing to target specific genes. Although the U.S. Food and Drug Administration (FDA) has approved multiple siRNA-based therapeutics, many biological barriers limit their use for treating diseases.
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What does gene silencing mean?
Gene silencing is a relatively new treatment technique that makes use of the body's natural processes to control disease by suppressing or 'silencing' specific genes that are associated with certain diseases.
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What is the mechanism of siRNA silencing?
The majority of the off-target gene silencing of siRNA is due to the partial sequence homology, especially within the 3'untranslated region (3'UTR), exists with mRNAs other than the intended target mRNA [158]. This mechanism is similar to the microRNA (miRNA) gene silencing effect.
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