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Preparation of Uniformly Isotope Labeled DNA Oligonucleotides for Spin Niddk Nih

Preparation of Uniformly Isotope Labeled DNA Oligonucleotides for Spin Niddk Nih

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[Music] oligonucleotides are used in a wide range of applications including genetic testing therapeutics and forensics in genetic research and testing the most common application of oligos is their use as primers for pcr polymerase chain reaction pcr is the technique of making many copies of a fragment or strand of dna to then generate thousands or millions of more copies for use in other downstream applications like plumbing or sequencing researchers use oligos that are anywhere between 20 to 35 bases long called primers to start copying or amplifying dna segments this dna primer is usually custom designed to match the target sequence of dna for copying oligonucleotides for therapeutic purposes have gained increased importance in the past 20 years different classes of oligonucleotide therapeutics exist depending on their mode of interaction with the target molecules oligonucleotides binding to mrna rely on a watson-crick-based pairing mechanism the goal is to identify sequences that a

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