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the mass spectrometer is made up of five main components the inlet the source the flight tube the magnet and the collector cups in this example i'm going to use co2 with the masses 44 45 and 46. the sample gases enter the mass spec through the inlet once in the source the co2 molecules are bombarded with high energy electrons knocking an electron off the co2 molecule and ionizing it the ions are then accelerated through the flight tube where they encounter a magnetic field that changes their flight path based on their atomic mass to charge ratio the lighter ions are deflected more than the heavier ions in this example you can see the mass 44 ions hit the leftmost collector cup and the heavier 45 and 46 hit the cups to the right as the ions hit the collector cups they generate an electrical signal that is proportional to the number of ions hitting the integrated signals from each collector cup can then be used to calculate a ratio of heavy to light isotopes you

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