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Expression and Introduction of Macromolecules into Cells in Sun Lab Med Nyu  Form

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a current focus of our lab of cellular imaging and macromolecular biophysics is the application and development of electron microscopy techniques for determining the 3d ultrastructure of eukaryotic cells and tissues one technique that we've been working with is serial block face scanning electromicroscopy which consists of an ultra microtone built into a specimen chamber of an sdm images of a black face are acquired by detecting the back scattered electron signal from heavy atom stained plastic embedded tissues and cells we've applied serial block face sem to image beta cells of mouse pancreatic islets of langerhans by measuring the ratio of the volumes of the immature granule cores to the mature granule cause it becomes feasible to determine the mass ratio of pro-insulin to insulin these results demonstrate that it is possible to build quantitative models of secretion pathways using the new generation of 3d em techniques

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