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People also ask
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What is the antisense RNA approach?
Antisense RNAs may be used to interfere with the phage lytic cycle by inhibiting the translation of phage-encoded genes essential for normal development. Mechanistically, antisense RNA hybridizes to the sense RNA strand and creates a translationally inactive double-stranded RNA (dsRNA) molecule.
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What are antisense RNA strategies for regulation of gene expression?
Antisense RNAs are utilized for gene regulation and specifically target mRNA molecules that are used for protein synthesis. The antisense RNA can physically pair and bind to the complementary mRNA, thus inhibiting the ability of the mRNA to be processed in the translation machinery.
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What is the concept of antisense therapy?
Treatment with antisense oligonucleotides. These are small pieces of DNA or RNA that can bind to specific molecules of RNA. This blocks the cell's ability to use the RNA to make a protein or work in other ways.
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How does antisense RNA affect translation?
Antisense RNA is believed to inhibit translation or induce degradation of mRNA through base pairing. Recently, many small noncoding RNAs (sRNAs) have been identified in E. coli (4-6). The majority of these RNA molecules function as antisense oligomers through base pairing with mRNAs (7-11).
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What is the antisense RNA technique?
Antisense RNA technology involves modifying RNA molecules to create a complimentary copy of the target messenger RNA (mRNA) in a reverse orientation. This complimentary copy, also called antisense RNA, can bind to the target mRNA, and regulate or inhibit the expression of the corresponding protein [20, 35].
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What is the molecular mechanism of antisense RNA technology?
The antisense RNA molecule is a unique DNA transcript consisting of 19–23 nucleotides, characterized by its complementary nature to mRNA. These antisense RNAs play a crucial role in regulating gene expression at various stages, including replication, transcription, and translation.
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How does antisense RNA inhibit gene expression?
'Post-transcriptional regulation:' e) AsRNA-mRNA duplex can either block ribosome from binding to the mRNA or recruit RNase H to degrade the mRNA. By this mechanism, asRNAs directly inhibit translation of the mRNAs.
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