Application for Environmental Laboratory Certification
Office of Environmental Laboratory Certification
A. Purpose of Application: Check one and complete the information.
__ Initial Certification (New Lab)
__ Additional Parameter Certification
__ Due to PT Failure
__ Recertification (Lab Previously Certified)
EPA Lab Code _ _ _ _ _ _ _
Assigned S.C. Laboratory I.D. # _ _ _ _ _
Old SC Laboratory I.D.# _ _ _ _ _
B. Laboratory Name:
C. Mailing Address
(P.O. Box or Number and Street)
(City)
(State)
(Zip Code)
(State)
(Zip Code)
(State)
(Zip Code)
D. Physical Address:
(Number and Street)
(City)
(County Code Number)
E. Billing Address (for Certification Fees):
(Billing Name)
(P.O. Box or Number and Street)
(City)
F. Laboratory Telephone Number:
DHEC 2802 (8/2012)
G. Laboratory FAX Number:
Page 1 of 33
H. Laboratory E-mail Address:
I. Type of Laboratory
__ Commercial
__ Federal
__ Industrial
___ Mobile
___ Municipal
___ State
J. Out-of-State Laboratories Only:
See out-of-state checklist for the required documentation. Only one State Certifying Authority can be designated for
each program below and the State Certifying Authority must have performed an on-site laboratory evaluation.
Safe Drinking Water Act: __________________________________________ Expiration Date: ___________
(Name of State Certifying Authority)
Clean Water Act: ________________________________________________ Expiration Date: ___________
(Name of State Certifying Authority)
Solid and Hazardous Waste: _______________________________________ Expiration Date: ___________
(Name of State Certifying Authority)
K. Laboratory Director: (Primary Contact) - Designate the person responsible for the laboratory operations.
Include a resume´ and/or qualifications.
Name and Title
Telephone Number
Extension
E-Mail Address:
Education:
Name of Institution:
___________________________________________________________________________________
___________________________________________________________________________________
Degree:
___________________________________________________________________________________
Major Field:
___________________________________________________________________________________
Certificates or Registrations Held:
___________________________________________________________________________________
___________________________________________________________________________________
Issuing Agency:
___________________________________________________________________________________
Date of Issue:
___________________________________________________________________________________
Experience (related to laboratory analysis):
___________________________________________________________________________________
___________________________________________________________________________________
___________________________________________________________________________________
L. General Certification Contact Person for Laboratory:
Name and Title
Telephone Number
DHEC 2802 (8/2012)
Extension
E-Mail Address:
Page 2 of 33
M. Laboratory Personnel: List all personnel involved in the laboratory operations. Please make copies of this form for
additional personnel. Resumes may be attached.
Name and Position Held:
____________________________________________________________________________________
Education and Experience:
____________________________________________________________________________________
____________________________________________________________________________________
____________________________________________________________________________________
License or Registration:
____________________________________________________________________________________
____________________________________________________________________________________
Primary Responsibilities in the Laboratory:
____________________________________________________________________________________
____________________________________________________________________________________
Name and Position Held:
____________________________________________________________________________________
Education and Experience:
____________________________________________________________________________________
____________________________________________________________________________________
____________________________________________________________________________________
License or Registration:
____________________________________________________________________________________
____________________________________________________________________________________
Primary Responsibilities in the Laboratory:
____________________________________________________________________________________
____________________________________________________________________________________
Name and Position Held:
____________________________________________________________________________________
Education and Experience:
____________________________________________________________________________________
____________________________________________________________________________________
____________________________________________________________________________________
License or Registration:
____________________________________________________________________________________
____________________________________________________________________________________
Primary Responsibilities in the Laboratory:
____________________________________________________________________________________
____________________________________________________________________________________
Name and Position Held:
____________________________________________________________________________________
Education and Experience:
____________________________________________________________________________________
____________________________________________________________________________________
____________________________________________________________________________________
License or Registration:
____________________________________________________________________________________
____________________________________________________________________________________
Primary Responsibilities in the Laboratory:
____________________________________________________________________________________
____________________________________________________________________________________
DHEC 2802 (8/2012)
Page 3 of 33
N. Safe Drinking Water Act Methodology:
Disinfection By-Products: Circle only the EPA-approved methodology that the laboratory is seeking certification to
Perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for
each parameter and method circled.
Parameter
Bromate
Bromide
Chlorite (Monthly/Daily)11
Methodology1
EPA
EPA 300.1 Rev. 1.0
EPA 317.0 Rev. 2.045
EPA 326.045
EPA 321.845,46
EPA 300.0 Rev. 2.1
EPA 300.1 Rev. 1.0
Standard Methods
Other
EPA 300.0 Rev. 2.1
EPA 300.1 Rev. 1.0
EPA 317.0 Rev. 2.0
EPA 326.0
EPA 327.0 Rev. 1.1
SM 4500-CIO2 E11
UV254
EPA 415.3 Rev.1.1
SM 5910 B
Inorganic-Demand: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
parameter and method circled.
49
Methodology1
Standard
EPA
Methods
Other
Total Organic Carbon/ Dissolved
SM 5310 B
Organic Carbon (TOC/DOC)47,48
EPA 415.3 Rev.1.1
SM 5310 C
SM 5310 D
Inorganic Mineral: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
parameter and method circled.
Parameter
Alkalinity
Methodology1
Standard
Methods
SM 2320 B
Calcium-Hardness
SM 3500-Ca D
Parameter
EPA
Chloride
Fluoride
Hydrogen-Ion Concentration (pH)
EPA 300. Rev. 2.1
EPA 300.1 Rev. 1.0
EPA 300. Rev. 2.1
EPA 300.1 Rev. 1.0
EPA 150.1
EPA 150.2
Specific Conductance
Sulfate
DHEC 2802 (8/2012)
Other
SM 4110B
SM 4500-CI-B
SM 4500-CI-D
SM 4110B
SM 4500-F- B,D
SM 4500-F- C
SM 4500-F- E
SM 4500-H+B
SM 2510 B
EPA 300. Rev. 2.1
EPA 300.1 Rev. 1.0
EPA 375.2
SM 4110B
SM 4500-SO42-F
SM 4500-SO42-C
SM 4500-SO42-D
SM 4500-SO42-E
Page 4 of 33
N. Safe Drinking Water Act Methodology:
Inorganic-Miscellaneous: Circle only the EPA-approved methodology that the laboratory is seeking certification to
perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for
each parameter and method circled.
Parameter
Asbestos
Chlorine Dioxide
Methodology1
EPA
EPA 100.1
EPA 100.2
EPA 327.0 Rev. 1.1
Standard Methods
Other
SM 4500-CIO2 C8
SM 4500-CIO2 D
SM 4500-CIO2 E
Color
- Visual
- Spectrophotometric (Tristimulus)
- Spectrophotometric (Plat. Cobalt)
Cyanide
(Manual Distillation Required)
SM 2120 B
SM 2120 C
EPA 335.4 Rev. 1.0
SM 4500-CN-C,E
SM 4500-CN- F
Cyanide- Amenable to Chlorination
SM 4500-CN-G31
Odor
Surfactants (MBAS)
SM 4500-CI D
SM 4500-CI E
SM 4500-CI F
SM 4500-CI G
SM 4500-CI H
SM 4500-CI I
SM 5540 C
Temperature
Kelada 0133
10-204-00-1-X34
OIA-167732
SM 2150 B
Residual Chlorine
NCASI-TB2539
SM 2550 B
Turbidity
DHEC 2802 (8/2012)
EPA 180.1 Rev. 2.0
SM 2130 B
Page 5 of 33
N. Safe Drinking Water Act Methodology:
Inorganic – Nutrient: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
parameter and method circled.
Methodology1
Parameter
EPA
EPA 300. Rev. 2.1
EPA 300.1 Rev. 1.0
EPA 353.2 Rev. 2.0
Nitrate-Nitrogen
Nitrate-Nitrite (NO3 + NO 2)
Nitrite-Nitrogen
EPA 300. Rev. 2.1
EPA 300.1 Rev. 1.0
EPA 353.2 Rev. 2.0
EPA 300. Rev. 2.1
EPA 300.1 Rev. 1.0
EPA 353.2 Rev. 2.0
Orthophosphate
EPA 300. Rev. 2.1
EPA 300.1 Rev. 1.0
EPA 365.1 Rev. 2.0
Phosphorus
Standard Methods
SM 4110 B
Other
SM 4500-NO3-F
SM 4500-NO3-E
SM 4500-NO3-D
SM 4110B
SM 4500-NO3-F
SM 4500-NO3-E
SM 4110B
SM 4500-NO3-F
SM 4500-NO3-E
SM 4500-NO2-B
SM 4110 B
SM 4500-P F
SM 4500-P E
SM 4110 B
SM 4500-P F
EPA 300.0 Rev. 2.1
EPA 365.1 Rev. 2.0
EPA 365.2
EPA 365.3 (1978)
SM 4500 P E
Inorganic – Residue: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate
certification from each parameter and method circled.
Methodology1
Parameter
EPA
Residue, Filterable (TDS)
DHEC 2802 (8/2012)
Standard Methods
SM 2540 C
Other
Page 6 of 33
N. Safe Drinking Water Act Methodology:
Inorganic – Trace Metal: Circle only the EPA-approved methodology that the laboratory is seeking certification to
perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for
each parameter and method circled.
Parameter
FLAA
GFAA/
GFAA/
ICP
ICP/
Other
Platform
Furnace
MS
SM1
EPA1
SM1
EPA1
SM1
EPA1
Aluminum
200.9
3113 B
Antimony
200.9
Arsenic
200.9
Barium
3111 D
3111 D
200.7
3120 B
200.8
200.5
3113 B
200.8
200.5
3113 B
200.8
200.5
3113 B
200.7
3120 B
200.8
200.5
3120 B
200.8
200.5
200.8
200.5
Beryllium
200.9
3113 B
200.7
Cadmium
200.9
3113 B
200.7
Calcium
3111 B
Chromium
200.7
3120 B
200.5
200.9
3113 B
200.7
3120 B
200.8
200.5
200.8
200.5
Copper
3111 B
200.9
3113 B
200.7
3120 B
Iron
3111 B
200.9
3113 B
200.7
3120 B
200.9
3113 B
Lead
Magnesium
3111 B
Manganese
3111 B
200.8
200.7
200.9
3113 B
3120 B
200.7
3120 B
3111 B
200.9
3113 B
200.9
Selenium
200.5
200.7
3120 B
200.7
Silver
3111 B
Sodium
3111 B
Thallium
200.9
3113 B
3120 B
200.8
EPA 245.1
EPA 245.2
200.5
200.5
200.5
200.8
200.7
SM 3112 B
4500 Si D
4500 Si E
4500 Si F
200.5
200.5
200.9
3111 B
200.5
3120 B
200.7
200.8
200.8
3113 B
Silica
Zinc
200.5
200.8
Mercury
Nickel
200.5
200.8
200.7
3120 B
200.8
200.5
The 20th Edition of Standard Methods is not an approved reference for these methods.
DHEC 2802 (8/2012)
Page 7 of 33
N. Safe Drinking Water Act Methodology:
Microbiology: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform. The
laboratory must be approved for a total coliform method and a fecal coliform or E. coli method. Out-of-state laboratories:
The State Certifying Authority’s certificate must reflect the appropriate certification for each parameter and method
circled.
Parameter
Methodology1
E.coli Enumeration (MF)
E.coli Enumeration (MPN)
SM 9222B/9222G
m-ColiBlue24®6
SM 9221B.1/9221F
Fecal Coliform (MPN)
SM 9221C, E
Fecal Coliform (MF)
SM 9222 D
Heterotrophic Bacteria
37
Total Coliform (Delayed Incubation)
SM 9215 B
SimPlate36
SM 9222 C
Total Coliform (MF)2
Fecal Coliform Confirmation
Total Coliform (MPN)
SM 9222B (M-endo medium)
SM 9221 E
SM 9221 B
Total Coliform (P-A)
E. coli Confirmation
Total Coliform/E. coli
(Presence/Absence)
SM 9221 D
SM 9221 F
SM 9223B Colilert3
Colisure® 4
m-ColiBlue24® 6
Colitag® 40
SM 9223B Colilert Quanti-Tray® 3
Total Coliform/E. coli (MPN)*
* Method can be used for reporting under the TCR, SWTR, and LT2 Rule.
LT2 Rule requires enumeration of E.Coli.
Trihalomethanes: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform. Outof-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each method
and organic contaminant circled.
EPA Methodology1
Parameter
Volatile Organics by P&T – GC/PID/Hall
GC
GC/MS
502.2
Purgeable Organics by Cap. Col. – GC/MS
524.2
Disinfection Byproducts & Chlor. Solvents GC-ECD
524.3
551.1
Volatiles (VOCs): Circle only the EPA-approved methodology that the laboratory is seeking certification to perform. Outof-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each method
and organic contaminant circled.
EPA Methodology1
Parameter
Volatile Organics by P&T – GC/PID/Hall
GC
GC/MS
502.2
Purgeable Organics by Cap. Col. – GC/MS
Disinfection Byproducts & Chlor. Solvents GC/ECD
DHEC 2802 (8/2012)
524.2
524.3
551.1
Page 8 of 33
N. Safe Drinking Water Act Methodology:
Synthetic Organic Chemicals (SOCs): Circle only the EPA-approved methodology that the laboratory is seeking
certification to perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate
certification for each method and organic contaminant circled.
EPA Methodology1
Analytical Method
Tetra-Octa-Chlor. Dioxins & Furans - HRGC/HRMS
GC
GC/MS
HPLC
1613B
EDB, DBCP, 1,2,3 TCP by Microext - GC/ECD
504.1
Organohalide Pest & PCBs by Microext - GC/ECD
50516
Phthalate and Adipate Esters - GC-PID
506
Nitrogen & Phosphorus Pesticides - GC/NPD
507
Chlorinated Pesticides by Liq-Liq Ext. - GC/ECD
508
Chlorinated Pesticides by LSE - GC/ECD
508.1
Screening for PCBs by Perchlorination - GC/ECD
508A14
Chlorinated Acids by Liq-Liq Ext. - GC/ECD
515.1
Chlorinated Acids by Liq-Solid Ext. - GC/ECD
515.2
Chlorinated Acids by Liq-Liq Ext. - GC/ECD
515.3
Chlorinated Acids by Liq-Liq Micro Ext - GC/ECD
515.4
555
Organic Compounds by Liq-Solid Ext. - GC/MS
525.2
N-Methylcarbamoyloximes & Carbamates - HPLC
531.1
531.2
547
Glyphosate - HPLC/Fluorescence Detector
Endothall - GC/MS
SM 6651
548.1
Diquat & Paraquat - HPLC/UV Detector
549.2
Polynuclear Arom. Hydroc. (PAHs) - HPLC/UV &
Fluores.
Disinfection ByProducts, Chlor. Solvents,Halogenated
Pest/Herb - GC/ECD
Haloacetic Acids & Dalapon by Ion Exchange Liq-Solid
Ext. – GC/ECD
Haloacetic Acids & Dalapon by Liq-Liq Ext. - GC-ECD
550
550.1
DHEC 2802 (8/2012)
551.1
552.1
552.2
552.3
SM 6251B
Page 9 of 33
N. Safe Drinking Water Act Methodology:
Inorganic – Radiological: Circle only the EPA-approved methodology that the laboratory is seeking certification to
perform. Out-of-state laboratories: The State Certifying Authority’s certification must reflect the appropriate certification
for each parameter and method circled.
EPA1
Standard
Parameter
Methodology
Methods1
131
Iodine
EPA 901.1
SM 7120
EPA 902.0
SM 7500-I B
SM 7500-I C
SM 7500-I D
Gross Alpha
EPA 900.0
SM 302 (13th)
SM 7110B
SM 7110C
Gross Beta
EPA 900.0
SM 302 (13th)
SM 7110B
226
Radium
EPA 903.0
SM 304 (13th)
SM 7500-Ra B
EPA 903.1
SM 305 (13th)
SM 7500-Ra C
228
Radium
EPA 904.0
SM 7500-Ra D
89
EPA 905.0
90
EPA 905.0
Tritium
EPA 906.0
Strontium
Strontium
Uranium
Radiochemical
Fluorometric
Alpha Spectrometry
ICP/MS
Gamma Emitters 43
DHEC 2802 (8/2012)
EPA 908.0
EPA 908.1
EPA 200.8
EPA 901.1
EPA 902.0
EPA 901.0
SM 303 (13th)
SM 7500-Sr B
SM 303 (13th)
SM 7500-Sr B
SM 306 (13th)
SM 7500-3H B
SM 7500-U B
SM 7500-U C (17th)
SM 7500-U C (18th & 19th)
SM 7120
SM 7500 Cs B
SM 7500 I B
Page 10 of 33
O. Clean Water Act Methodology
Microbiology: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform. Out-ofstate laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each parameter
and method circled.
Parameter
Enterococci (MF)
Methodology1
EPA
EPA 1600 (2009)
Standard Methods
Enterolert17
Enterococci (MPN)
Fecal Coliform (MF)
Fecal Coliform (MPN)
Fecal Coliform (MPN)
Biosolids
Biosolids Preparation
Other
SM 9222 D-1997
EPA 1680 (2010)24
EPA 1681 (2006)24
SM 9221 C E-2006
SM 9221 C E-2006 (Biosolids
Prep. Certification Required)
Total Coliform (MF)
SM 9221 B-2006
Fecal Streptococci (MF)
SM 9230 C-2007
Fecal Streptococci (MPN)
EPA/625/R-92/013 App F42
SM 9222 B-1997
Total Coliform (MPN)
Colilert® 18 ATP QuantiTray®
SM 9230 B-2007
E.Coli (MF)
m-ColiBlue24®6
EPA 1603 (2009)
E. Coli (MPN)
SM 9223B-2004
SM 9221B.1-2006/9221F-2006
Colilert®/Colilert-18®
Taxonomy: Circle the parameter that the laboratory is seeking certification to perform.
Parameter
Methodology
Freshwater Fish
Key/Reference
Freshwater Macroinvertebrates
Key/Reference
Ichthyoplankton
Key/Reference
Macrophytes
Key/Reference
Marine/Estuarine Fish
Key/Reference
Marine/Estuarine Macroinvertebrates
Key/Reference
Periphyton
Key/Reference
Phytoplankton
Key/Reference
Zooplankton
Key/Reference
DHEC 2802 (8/2012)
Page 11 of 33
O. Clean Water Act Methodology:
Toxicity Testing: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Laboratories must be certified for pH, DO, alkalinity, specific conductance, hardness, and residual chlorine in order to
become certified for toxicity. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the
appropriate certification for each parameter and method circled. If the certificate does not state that the laboratory’s
certification includes pH, DO, alkalinity, specific conductance, hardness, and residual chlorine; a letter from the State
Certifying Authority stating that these parameters have been evaluated, must be submitted with this application.
Parameter
Methodology
Ceriodaphnia dubia
Acute Toxicity - Ceriodaphnia dubia
EPA 2002.0 (2002)
Chronic Toxicity - Ceriodaphnia dubia
EPA 1002.0 (2002)
Mysidopsis bahia (Americamysis bahia)
Acute Toxicity - Mysidopsis bahia
EPA 2007.0 (2002)
Chronic Toxicity - Mysidopsis bahia
EPA 1007.0 (2002)
Pimephales promelas
Acute Toxicity - Pimephales promelas
EPA 2000.0 (2002)
Chronic Toxicity - Pimephales promelas
EPA 1000.0 (2002)
Daphnia ambigua
Acute Toxicity - Daphnia ambigua
EPA 2002.0 (2002)
Chronic Toxicity - Daphnia ambigua
EPA 1002.0 (2002)
Cyprinodon variegates
Acute Toxicity - Cyprinodon variegates
EPA 2004.0 (2002)
Chronic Toxicity - Cyprinodon variegates
EPA 1004.0 (2002)
Menidia beryllina
Acute Toxicity – Menidia beryllina
EPA 2006.0 (2002)
Chronic Toxicity – Menidia beryllina
EPA 1006.0 (2002)
Inorganic – Biological Examinations: Circle only the EPA-approved methodology that the laboratory is seeking
certification to perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate
certification for each parameter and method circled where applicable.
Parameter
Methodology1
EPA
Standard Methods
Biomass – Plankton
SM 10200 I-2011
Biomass – Peripyton (Dry Weight)
Chlorophyll a
DHEC 2802 (8/2012)
Other
SM 10300 C-2010
EPA 445.0 Rev. 1.2
41
SM 10200 H-2011
Page 12 of 33
O. Clean Water Act Methodology:
Inorganic – Demand: Circle only the EPA-approved methodology that the laboratory is seeking to perform. Out-of-state
laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each parameter and
method circled.
Methodology1
Parameter
EPA
Biochemical Oxygen Demand (BOD5)
(DO Certification Required)
Carbonaceous BOD (CBOD5)
(DO Certification Required)
Chemical Oxygen Demand
Standard Methods
SM 5210 B-2011
Other
SM 5210 B-2011
EPA 410.3 (Rev. 1978)
EPA 410.4 Rev.2.0 (1993)
Dissolved Oxygen (DO)
SM 5220 B-2011
SM 5220 C-2011
SM 5220 D-2011
SM 4500-O C-2011
SM 4500-O G-2011
Hach 8000
ASTM D888-09(A)
ASTM D888-09(B)
ASTM D888-09(C)
Hach 10360 Rev. 1.2
Total Organic Carbon (TOC)
SM 5310 B-2011
SM 5310 C-2011
SM 5310 D-2011
Inorganic – Mineral: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
parameter and method circled.
Methodology1
Parameter
EPA
Acidity
Alkalinity
Standard Methods
SM 2310 B-2011(4a)
Other
SM 2320 B-2011
EPA 310.2 ( Rev. 1974)
EPA 300.0 Rev 2.1 (1993)
EPA 300.1 Rev 1.0 (1997)
Chloride
Fluoride
EPA 300.0 Rev. 2.1 (1993)
EPA 300.1 Rev 1.0 (1997)
Manual distillation required:
SM 4500-F- D-2011
SM 4500-F- C-2011
SM 4500-F- E-2011
Followed by:
Hardness, Total (CaCO3)
EPA 130.1 (Rev. 1971)
SM 2340 C-2011
SM 2340 B-2011(calc.)
SM 4500-H+ B-2011
Hydrogen-Ion Concentration (pH)
Specific Conductance
Sulfate
EPA 150.2 (Rev. 1982)
EPA 120.1(Rev. 1982)
EPA 300.0 Rev. 2.1 (1993)
EPA 300.1Rev 1.0 (1997)
EPA 375.2 Rev. 2.0 (1993)
DHEC 2802 (8/2012)
SM 4110 B-2011
SM 4110 C-2011
SM 4500-CI- B-2011
SM 4500-CI- E-2011
SM 4500-Cl- D-2011
SM 4500-CI- C-2011
SM 4110 B-2011
SM 4110 C-2011
SM 4500-F- B-2011
SM 2510 B-2011
SM 4110 B-2011
SM 4110 C-2011
SM 4500-SO42- C-2011
SM 4500-SO42- D-2011
SM 4500-SO42- E-2011
SM 4500-SO42- F-2011
SM 4500-SO42- G-2011
Page 13 of 33
O. Clean Water Act Methodology
Inorganic – Miscellaneous: Circle only the EPA-approved methodology that the laboratory is seeking certification to
perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for
each parameter and method circled.
Parameter
Absorbable Organic
Halides(AOX)
Bromide
Methodology1
EPA
EPA 1650C
EPA 300.0 Rev.2.1 (1993)
EPA 300.1 Rev 1.0 (1997)
Color
ADMI (Tristimulus)
Visual(Platinum Cobalt)
Spectrophotometric
(Plat.Cobalt)
Cyanide, Total
Distillation with MgCI2 required:
Followed by:
Other
SM 4110 B-2011
SM 4110 C-2011
SM 4110 D-2011
SM 4140 B-2011
SM 2120 E-2011
SM 2120 B-2011
SM 2120 C-2011
NCASI TB 253 (1971)
Kelada-0133
EPA 335.4 Rev.1.0 (1993)
SM 4500-CN-B-2011
SM 4500-CN-C-2011
10-204-00-1-X34
EPA 335.4 Rev.1.0 (1993)
SM 4500-CN-D-2011
SM 4500-CN-E-2011
SM 4500-CN-F-2011
SM 4500-CN-G-201131
10-204-00-1-X34
Cyanide, Available (Amenable)
(Must be certified for a Total
Cyanide method)
Cyanide, Free
Oil and Grease
Standard Methods
Kelada-0133
OIA-167732
OIA-167732
EPA 1664B (2010)
Phenols
Manual distillation required:
Followed by:
EPA 420.1 Rev. 1978
EPA 420.4 Rev.1.0 (1993)
EPA 420.1 Rev. 1978
EPA 420.4 Rev.1.0 (1993)
Residual Chlorine
SM 5530B-2005
SM 5530D-2005
SM 4500-CI B-2011
SM 4500-CI C-2011
SM 4500-CI D-2011
SM 4500-CI E-2011
SM 4500-CI F-2011
SM 4500-CI G-2011
Sulfide
Sulfite
SM 4500-S2- B-2011
SM 4500-S2- C-2011
SM 4500-S2- D-2011
SM 4500-S2- F-2011
SM 4500-S2- G-2011
SM 4500-SO32- B-2011
Surfactants (MBAS)
SM 5540 C-2011
Temperature
SM 2550 B-2011
Sample Pretreatment
Followed by:
Turbidity
DHEC 2802 (8/2012)
EPA 180.1 Rev.2.0 (1993)
SM 2130 B-2011
Page 14 of 33
O. Clean Water Act Methodology
Inorganic – Nutrient: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
parameter and method circled.
Parameter
Ammonia-Nitrogen
Manual distillation
required:
Followed by:
Kjeldahl-Nitrogen (TKN)
Manual digestion and
distillation required:
Followed by
Manual distillation not
required:
Nitrate-Nitrogen (NO3)
Nitrate-Nitrite Nitrogen
(NO3 + NO2)
Methodology1
EPA
EPA 350.1 Rev. 2.0 (1993)
EPA 350.1 Rev. 2.0 (1993)
EPA 350.1 Rev. 2.0 (1993)
EPA 351.1 (Rev. 1978)
EPA 351.2 Rev. 2.0 (1993)
EPA 352.1
EPA 300.0 Rev.2.1 (1993)
EPA 300.1 Rev 1.0 (1997)
EPA 353.2 Rev. 2.0 (1993)
EPA 300.0 Rev.2.1 (1993)
EPA 300.1Rev 1.0 (1997)
Nitrite-Nitrogen (NO2)
Orthophosphate
EPA 353.2 Rev. 2.0 (1993)
EPA 300.0 Rev.2.1 (1993)
EPA 300.1Rev 1.0 (1997)
EPA 365.1 Rev. 2.0 (1993)
EPA 365.3 (Rev. 1978)
EPA 300.0 Rev.2.1 (1993)
EPA 300.1 Rev 1.0 (1997)
Phosphorus
Total Organic Nitrogen
DHEC 2802 (8/2012)
EPA 365.4 (Rev. 1974)
EPA 365.1 Rev. 2.0 (1993)
EPA 365.3 (Rev. 1978)
EPA 200.7 Rev. 4.4 (1994)
EPA TKN-NH3(N)
Standard Methods
Other
SM 4500-NH3 B
SM 4500-NH3 C-2011
SM 4500-NH3 D-2011
SM 4500-NH3 E-2011
SM 4500-NH3 F-2011
SM 4500-NH3 G-2011
SM 4500-NH3 H-2011
SM 4500-Norg B-2011
SM 4500-Norg C-2011
SM 4500-NH3 B
SM 4500-NH3 C-2011
SM 4500-NH3 D-2011
SM 4500-NH3 E-2011
SM 4500-NH3 F-2011
SM 4500-NH3 G-2011
SM 4500-NH3 H-2011
SM 4500-Norg D-2011
SM 4110 B-2011
SM 4110 C-2011
SM 4140 B-2011
SM 4500-NO3 D-2011
SM 4500-NO3- H-2011
SM 4500-NO3- E-2011
SM 4500-NO3- F-2011
SM 4110 B-2011
SM 4110 C-2011
SM 4500-NO2- B-2011
SM 4500-NO3- E-2011
SM 4500-NO3- F-2011
SM 4110 B-2011
SM 4110 C-2011
SM 4500-P F-2011
SM 4500-P E-2011
NO3-NO2 Minus NO2
SM 4110 B-2011
SM 4110 C-2011
Digestion:
SM 4500-P B(5)-2011
Followed by:
SM 4500-P F-2011
SM 4500-P E-2011
SM 3120 B-2011
Page 15 of 33
O. Clean Water Act Methodology:
Inorganic – Trace Metal: Circle only the EPA-approved methodology that the laboratory is seeking certification to
perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for
each parameter and method circled.
STGFAA
GF
ICP
ICP/MS
Other
Parameter
Aluminum
EPA1
200.9
SM1
3113 B-2010
EPA1
200.7
EPA1
200.8
EPA1
200.5
Antimony
200.9
3113 B-2010
200.7
200.8
200.5
Arsenic
200.9
3113 B-2010
200.7
200.8
200.5
3113 B-2010
200.7
200.8
200.5
3113 B-2010
200.7
200.8
200.5
Barium
Beryllium
200.9
Boron
Cadmium
200.7
200.9
3113 B-2010
Calcium
Chromium
200.7
200.5
200.8
200.7
200.9
3113 B-2010
200.7
SM1
200.5
200.5
200.8
Chromium VI
200.5
218.6 Rev. 3.3(1994)
Cobalt
200.9
3113 B-2010
200.7
200.8
200.5
Copper
200.9
3113 B-2010
200.7
200.8
SM 3500-Cr C-2011
SM 3500-Cr B-2011
200.5
Gold
231.2 (Rev. 1978)
Iridium
235.2 (Rev. 1978)
Iron
200.9
3113 B-2010
200.7
Lead
200.9
3113 B-2010
200.7
Magnesium
Manganese
200.5
200.8
200.7
200.9
3113 B-2010
200.7
200.5
200.8
Mercury
200.5
1631E
245.1 Rev. 3.0 (1994)
245.2 (Rev. 1974)
245.7 Rev. 2.0 (2005)
EPA 1669
Sampling for
Low-Level
Metals
Molybdenum
Nickel
200.5
3113 B-2010
200.9
200.7
200.8
200.5
3113 B-2010
200.7
200.8
3112B-2011
200.5
Osmium
252.2
Palladium
253.2
Platinum
255.2
Potassium
200.7
Rhodium
265.2
Ruthenium
267.2
Selenium
200.9
3113 B-2010
Silver
DHEC 2802 (8/2012)
200.9
3113 B-2010
200.8
200.5
200.7
Silica
200.7
200.8
200.5
200.7
200.8
200.5
Page 16 of 33
O. Clean Water Act Methodology:
Inorganic – Trace Metal (continued): Circle the EPA-approved methodology that the laboratory is seeking certification to
perform. Out-of-state laboratories: The State Certifying Authority’s Certificate must reflect the appropriate certification
for each parameter and method circled.
STGFAA
GF
ICP
ICP/MS
EPA1
SM1
EPA1
200.7
EPA1
200.8
EPA1
200.5
Thallium
200.9
3113 B-2010
200.7
200.8
Tin
200.9
3113 B-2010
200.7
200.8
279.2
200.5
200.5
200.7
200.8
283.2
200.7
200.8
200.5
200.7
200.8
289.2
200.5
Parameter
Sodium
Titanium
Vanadium
3113 B-2010
Zinc
Other
SM1
Inorganic – Radiological: Circle only the EPA-approved methodology that the laboratory is seeking certification to
perform. Out-of-state laboratories: The State Certifying Authority’s certification must reflect the appropriate certification
for each parameter and method circled.
Alpha-Total
EPA1
Methodology
EPA 900.0
Beta-Total
EPA 900.0
SM 7110B-2000
226
EPA 903.0
EPA 903.1
SM 7500-Ra B-2001
SM 7500-Ra C-2001
Parameter
Standard
Methods1
SM 7110B-2000
Radium
Inorganic – Residue: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
parameter and method circled.
Methodology1
Parameter
Residue, Filterable (TDS)
EPA
Standard Methods
SM 2540 C-2011
Residue, Non-filterable (TSS)
SM 2540 D-2011
Residue, Settleable (SS)
SM 2540 F-2011
Residue, Total (TS)
SM 2540 B-2011
Residue, Volatile (VS)
Total, Fixed, & Volatile Solids
DHEC 2802 (8/2012)
EPA 160.4
44
Other
SM 2540 E-2011
SM 2540 G-2011
Page 17 of 33
O. Clean Water Act Methodology:
Organic Analyses: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
parameter and method circled. Include a list of analytes for each method.
Parameter
Methodology1
GC
GC/MS
HPLC
Pesticides & PCBs
Organochlorine Pesticides & PCBs
EPA 60815
Organophosphate Pesticides
SM 6630 C
Herbicides
Chlorinated Phenoxy Acid Herbicides
SM 6640 B
EPA 615
Volatiles
Volatile Organics by Isotope Dilution –GC/MS
EPA 1624B
EPA 1624C30
EPA 1666A
VOCs by Isotope Dilution – GC/MS
VOCs by GC-FID
EPA 1671A
Purgeable Halocarbons –GC/Hall
EPA 601
Purgeable Aromatics – GC/PID
EPA 602
Acrolein & Acrylonitrile – GC/FID
EPA 603
Purgeables – GC/MS
EPA 624
Semivolatiles
Phenols – GC/FID
EPA 604
Benzidines –HPLC
EPA 605
Phthalate Esters – GC/ECD
EPA 606
Nitrosamines – GC/NPD
EPA 607
EPA 609
Nitroaromatics & Isophorone – GC/FID/ECD
Polynuclear Aromatic Hydrocarbons (PAHs) – GC/FID or HPLC
EPA 610
Haloethers – GC/Hall
EPA 611
Chlorinated Hydrocarbons – GC/ECD
EPA 612
Base Neutrals & Acids –GC/MS
SVO by Isotope –GC/MS
EPA 610
EPA 625
EPA 1625B
EPA 1625C30
EPA 1653A
Formaldehyde, Isobutyraldehyde, and Furfural by HPLC
EPA 1667A
Dioxins & Furans
Tetra-Octa-Chlorinated Dioxins & FuransHRGC/HRMS
EPA 1613B
2,3,7,8-Tetrachloridibenzo-p-Dioxin
EPA 613
DHEC 2802 (8/2012)
Page 18 of 33
P. Solid and Hazardous Waste Methodology:
Inorganic – Trace Metal: Circle only the EPA-approved methodology that the laboratory is seeking certification to
perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for
each parameter and method circled.
Cold
Other
Parameter
FLAA
GFAA
Hydride
ICP
ICP/MS
Colorimetric
Vapor
Aluminum
7000B
6010C
6020A
Antimony
Arsenic
7000B
6010C
6020A
6010C
6020A
Barium
7000B
7010
6010C
6020A
Beryllium
7000B
7010
6010C
6020A
Cadmium
7000B
7010
6010C
6020A
6800
Calcium
Chromium VI
7000B
6010C
6020A
Chromium
7000B
7010
6010C
6020A
6800
7195
7197
7198
7199
6800
Cobalt
7000B
7010
6010C
6020A
Copper
7000B
7010
6010C
6020A
6800
Iron
7000B
7010
6010C
6020A
6800
Lead
7000B
7010
6010C
6020A
6800
Lithium
7000B
6010C
Magnesium
7000B
6010C
6020A
6800
Manganese
7000B
6010C
6020A
7010
7010
7062
7061A
7062
6800
6800
7196A
7010
7470A
7471B
Mercury
7473
7474
6800
6800
Molybdenum
7000B
7010
6010C
Nickel
7000B
7010
6010C
6020A
6800
Osmium
7000B
Potassium
Selenium
7000B
6010C
6020A
6800
6010C
6020A
6800
6010C
6020A
6800
6020A
7010
Silica
7741A
7742
6010C
Silver
7000B
Sodium
7000B
6010C
Strontium
7000B
6010C
Thallium
7000B
Tin
7000B
Vanadium
7000B
7010
Zinc
7000B
7010
DHEC 2802 (8/2012)
7010
7010
6010C
6800
6020A
6800
6010C
6020A
6800
6010C
6020A
6800
6010C
Page 19 of 33
P. Solid and Hazardous Waste Methodology:
Inorganic – Trace Metal: Circle only the EPA-approved methodology that the laboratory is seeking certification to
perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for
each parameter and method circled.
Methodology1
Parameter
EPA
Field Portable X-Ray Fluorescence Spectrometry for the
Determination of Elemental Concentrations in Soil and Sediment
Standard Methods
Other
EPA 6200
Microbiology: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform. Out-ofstate laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each parameter
method circled.
Methodology1
Parameter
EPA
Standard Methods
Fecal Coliform (MF)
Fecal Coliform (MPN)
SM 9221 E
Fecal Streptococci (MF)
SM 9230 C
Fecal Streptococci (MPN)
Other
SM 9222 D
SM 9230 B
Salmonella (MF)
SM 9260 D2
Salmonella (MPN)
SM 9260 D1
Total Coliform (MPN)
EPA 9131
Total Coliform (MF)
EPA 9132
Inorganic – Hazardous Waste Characteristics: Circle only the EPA-approved methodology that the laboratory is seeking
certification to perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate
certification for each parameter method circled.
Parameter
EPA
Corrosivity
Towards steel
PH (See Hydrogen-Ion Conc./Method 9040B Under Minerals)
EPA 1120
EP Toxicity Test
Ignitability
Pensky Martens
Setaflash
Other
EPA 1110A
Dermal Corrosion
Methodology1
Standard
Methods
EPA 1310B
Ignitability of Solids
EPA 1010A
EPA 1020B
EPA 1030
Paint Filter Liquids Test
EPA 9095B
Liquid Release Test (LRT) Procedure
EPA 9096
Multiple Extraction Procedure
EPA 1320
Synthetic Precipitation Leaching Proc.
TCLP – Tox. Char. Leach. Proc. – Bottle Ext.
TCLP – Tox. Char. Leach. Proc. – Zero Head
Test Method for Oxidizing Solids
Test Methods to Determine Substances Likely to Spontaneously
Combust
EPA 1312
EPA 131125
EPA 131126
EPA 1040
DHEC 2802 (8/2012)
EPA 1050
Page 20 of 33
P. Solid and Hazardous Waste Methodology
Inorganic – Mineral: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
parameter and method circled.
Parameter
Chloride
Fluoride
Hydrogen-Ion Concentration (pH) (Corrosivity)
Hydrogen-Ion Concentration (solid)
Specific Conductance
Sulfate
EPA
EPA 9212
EPA 9250
EPA 9251
EPA 9253
EPA 9056A
EPA 6500
EPA 9214
EPA 9056A
EPA 6500
EPA 9040C
EPA 9045D
Methodology1
Standard
Methods
Other
EPA 9050A
EPA 9035
EPA 9036
EPA 9038
EPA 9056A
EPA 6500
Inorganic – Nutrient: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
parameter and method circled.
Parameter
Nitrate-Nitrogen
Nitrite-Nitrogen
Orthophosphate
Methodology1
EPA
EPA 9210A
EPA 9056A
EPA 6500
EPA 9056A
EPA 9216
EPA 6500
EPA 9056A
EPA 6500
Standard Methods
Other
Inorganic – Demand: Circle the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
parameter and method circled.
Parameter
Total Organic Carbon (TOC)
DHEC 2802 (8/2012)
Methodology1
EPA
Standard Methods
Other
EPA 9060A
Page 21 of 33
P. Solid and Hazardous Waste Methodology
Inorganic – Miscellaneous: Circle only the EPA-approved methodology that the laboratory is seeking certification to
perform. Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for
each parameter and method circled.
Parameter
Methodology1
EPA
Bomb Preparation Method
Bromide
Ion Chromatography
Electrode
Capillary Ion Electrophoresis
EPA 9090A
Cyanide (Distillation)
Cyanide
Spectrophotometric, Automated
Titrimetric & Manual Spectrophotometric27
Electrode27
EPA 9010C
Cyanide Amenable to Chlor. (Distillation)
Cyanide Amen. To Chlorination
Spectrophotometric, Automated
Titrimetric & Manual Spectrophotometric27
Electrode27
EPA 9010C
Cyanide Extraction for Solids and Oils
EPA 9013
Extract. Proc. For Oily Wastes
EPA 1330A
Extract. Organic Halides in Solids (EOX)
EPA 9023
Intrinsic Permeability
Oil and Grease
Other
EPA 9056A
EPA 9211
EPA 6500
Comp. Test (Wastes & Mem. Liners)
Standard Methods
EPA 5050
EPA 9100
EPA 9070A
EPA 9071B
EPA 9012B
EPA 9014
EPA 9213
EPA 9012B
EPA 9014
EPA 9213
Phenolics, Total Recoverable
Manual, Spectrophotometric
Colorimetric, Automated
Spectrophotometric, MBTH
EPA 9065
EPA 9066
EPA 9067
Purgeable Organic Halides (POX)
EPA 9021
Saturated Hydraulic Conductance
EPA 9100
Saturated Leachate Conductance
EPA 9100
Sulfides, Extractable
EPA 9031
Sulfides, Acid Soluble & Insoluble (Distillation)
Sulfides, Acid Soluble & Insoluble
Titrimetric27
Electrode27
Total Chlorine in New and Used Petroleum Products
by X-Ray Fluorescence Spectrometry (XRF)
Total Chlorine in New and Used Petroleum
Products by OCM
Total Chlorine in New and Used Petroleum
Products (Field Test Kit Methods)
Total Organic Halides (TOX)
Determination of Water in Waste Materials by Karl
Fischer Titration
Determination of Water in Waste Materials by
Quantitative Calcium Hydride Reaction
EPA 9030B
DHEC 2802 (8/2012)
EPA 9034
EPA 9215
EPA 9075
EPA 9076
EPA 9077
EPA 9020B
EPA 9000
EPA 9001
Page 22 of 33
P. Solid and Hazardous Waste Methodology:
Organic Analyses: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
method and organic contaminant circled. Include a list of analytes for each analytical and sample preparation
method.
Parameter
Pesticides and PCBs
Organochlorine Pesticides by GC: Capillary
Column
EPA1 Methodology
Extraction/Preparation Method28
EPA 8081B
EPA 3510C
EPA 3520C
EPA 3535A
EPA 3540C
EPA 3541
EPA 3545A
EPA 3546
EPA 3550C
EPA 3562
EPA 3580A
Compound-Independent Elemental
Quantitation of Pesticides by GC/AED (Atomic
Emission Detection)
EPA 8085
Organophosphorus Pesticides by GC:
Capillary Column
EPA 8141B
Polychlorinated Biphenyls by GC15
EPA 8082A
EPA 3510C
EPA 3520C
EPA 3535A
EPA 3540C
EPA 3541
EPA 3545A
EPA 3546
EPA 3550C
EPA 3580A
EPA 3510C
EPA 3520C
EPA 3535A
EPA 3540C
EPA 3541
EPA 3545A
EPA 3546
EPA 3550C
EPA 3580A
EPA 3510C
EPA 3520C
EPA 3535A
EPA 3540C
EPA 3541
EPA 3545A
EPA 3546
EPA 3550C
EPA 3562
EPA 3580A
Herbicides
Chlorophenoxy Acid Herbicides by GC
EPA 8151A
Volatiles
Nonhalogenated Volatile Organics
EPA 8015C
TPH – Low Boiling Point (GRO)
EPA 8015C (GRO)
DHEC 2802 (8/2012)
EPA 5021
EPA 5030B
EPA 5031
EPA 5032
EPA 5035
EPA 3585
EPA 5030B
EPA 5035
EPA 3585
Page 23 of 33
P. Solid and Hazardous Waste Methodology:
Organic Analyses: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
method and organic contaminant circled. Include a list of analytes for each analytical and sample preparation
method.
Parameter
EPA1 Methodology
Extraction/Preparation Method
Volatiles
Volatiles by GC/Hall/PID
EPA 8021B
Volatile Organics by GC/MS: Capillary
Column
EPA 8260B
Volatile Organics by Vacuum Distilllation in
Combination with GC/MS (VD/GC/MS)
Semivolatiles
EPA 8261
Acetonitrile by GC-NPD
EPA 8033
Acrylamide, Acrylonitrile, & Acrolein by HPLC
EPA 8316
Acrylamide by GC
EPA 8032A
Acrylonitrile by GC
Base Neutrals & Acids by GC/MS:Capillary Column
EPA 8031
EPA 8270D
Base Neutrals & Acids by GC/FT-IR
EPA 8410
Carbonyl Compounds by HPLC
Chlorinated Hydrocarbons by GC:Capillary Column
EPA 8315A
EPA 8121
EDB & DBCP by Microextraction/GC
Extractable Nonvolatiles by HPLC/TS/MS
EPA 8011
EPA 8321B
DHEC 2802 (8/2012)
EPA 5021
EPA 5030B
EPA 5032
EPA 5035
EPA 3585
EPA 5021
EPA 5030B
EPA 5031
EPA 5032
EPA 5035
EPA 3585
EPA 3510C
EPA 3520C
EPA 3535A
EPA 3540C
EPA 3541
EPA 3545A
EPA 3546
EPA 3550C
EPA 3560
EPA 3561
EPA 3580A
EPA 3510C
EPA 3520C
EPA 3535A
EPA 3540C
EPA 3541
EPA 3545A
EPA 3550C
EPA 3560
EPA 3561
EPA 3580A
EPA 3510C
EPA 3520C
EPA 3540C
EPA 3541
EPA 3550C
EPA 3580A
BASED ON ANALYTE
Page 24 of 33
P. Solid and Hazardous Waste Methodology:
Organic Analyses: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
method and organic contaminant circled. Include a list of analytes for each analytical and sample preparation
method.
EPA1
Extraction/Preparation Method
Parameter
Methodology
Semivolatiles
Extractable Nonvolatiles by HPLC/PB/MS
Haloethers by GC
EPA 8325
EPA 8111
N-Methylcarbamates
Nitroaromatics & Cyclic Ketones by GC
EPA 8318A
EPA 8091
Nitroglycerine by HPLC
EPA 8332
Nitroaromatics, Nitramines by HPLC
EPA 8330A
Nitrosamines by GC
EPA 8070A
EPA 3510C
EPA 3520C
EPA 3540C
EPA 3541
EPA 3545A
EPA 3550C
Explosives by GC
EPA 8095
EPA 3535
EPA 8330-EXT (Soil extraction)
PAHs & PCBs by TE/GC/MS
Pentachlorophenol (PCP) by UV-Induced
Colorimetry
Phenols by GC
EPA 8275A
Phthalate Esters by GC: Capillary Column
EPA 8061A
DHEC 2802 (8/2012)
BASED ON ANALYTE
EPA 3510C
EPA 3520C
EPA 3540C
EPA 3541
EPA 3550C
EPA 3510C
EPA 3520C
EPA 3540C
EPA 3541
EPA 3545A
EPA 3550C
EPA 3580A
EPA 8540
EPA 8041A
EPA 3510C
EPA 3520C
EPA 3535A
EPA 3540C
EPA 3541
EPA 3545A
EPA 3546
EPA 3550C
EPA 3580A
EPA 3510C
EPA 3520C
EPA 3535A
EPA 3540C
EPA 3541
EPA 3545A
EPA 3546
EPA 3550C
EPA 3580A
Page 25 of 33
P. Solid and Hazardous Waste Methodology
Organic Analyses: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
method and organic contaminant circled. Include a list of analytes for each analytical and sample preparation
method.
Parameter
EPA1 Methodology
Extraction/Preparation Method
Semivolatiles
Polynuclear Aromatic Hydrocarbons by GC/FID
Polynuclear Aromatic Hydrocarbons by HPLC
Tetrazine Reverse Phase by HPLC
TPH – High Boiling Point (DRO)
EPA 8100
EPA 8310
EPA 8331
EPA 8015C (DRO)
EPA 3510C
EPA 3520C
EPA 3540C
EPA 3541
EPA 3545A
EPA 3546
EPA 3550C
EPA 3561
EPA 3580A
EPA 3510C
EPA 3520C
EPA 3540C
EPA 3541
EPA 3545A
EPA 3546
EPA 3550C
EPA 3561
EPA 3580A
EPA 3510C
EPA 3520C
EPA 3535A
EPA 3540C
EPA 3541
EPA 3545A
EPA 3546
EPA 3550C
EPA 3560
EPA 3561
EPA 3580A
Dioxin & Dibenzofurans
PCDDs/PCDFs by HRGC/LRMS
EPA 8280B
PCDDs/PCDFs by HRGC/HRMS
EPA 8290A
DHEC 2802 (8/2012)
Page 26 of 33
P. Solid and Hazardous Waste Methodology:
Organic Analyses: Circle only the EPA-approved methodology that the laboratory is seeking certification to perform.
Out-of-state laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each
method and organic contaminant circled.
Parameter
EPA1 Methodology
Extraction/Preparation Method
Infared Methods
Fourier Transform Infrared by GC/FT-IR
Bis(2-chloroethyl) Ether & Hydrolysis by GC/FT-IR
Tot, Recoverable Petro. Hydrocarbons
EPA 8410
EPA 8430
EPA 8440
Immunoassay Methods
Immunoassay
Pentachlorophenol by Immunoassay
2,4-Dichlorophenoxyacetic Acid by Imm.
Polychlorinated Biphenyls by Imm.
Soil Screening for TPH by Imm.
Soil Screening for PAHs by Imm.
Soil Screening for Toxaphene by Imm.
Soil Screening for Chlordane by Imm.
Soil Screening for DDT by Imm.
EPA 4000
EPA 4010A
EPA 4015
EPA 4020
EPA 4030
EPA 4035
EPA 4040
EPA 4041
EPA 4042
EPA 4050
EPA 4051
EPA 4670
TNT Explosives in Soil by Imm.
Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)
Triazine Herbicides as Atrazine in Water
Miscellaneous Screening Methods
Headspace
Hexadecane Ext. & Screening of Purg.
Trinitrotoluene (TNT) in Soil (Color.)
Polychlorinated Biphenyls in Soil
Polychlorinated Biphenyls in Trans. Oil
Volatile Organics in Soil
Extracts of Environmental Samples for Planar
Organic Compounds (PAHs, PCBs,
PCDDs/PCDFs) by a Reporter Gene on a Human
Cell Line
Colorimetric Screening for RDX and HMX in Soil
Total Volatile Organic Halides in Water
EPA 5021
EPA 3820
EPA 8515
EPA 9078
EPA 9079
EPA 3815
EPA 4425
EPA 8510
EPA 8535
Q. Shellfish Waters and Meats Methodology:
Microbiology: Circle on the EPA-approved methodology that the laboratory is seeking certification to perform. Out-ofstate laboratories: The State Certifying Authority’s certificate must reflect the appropriate certification for each parameter
and method circled.
Methodology1
Parameter
EPA
Standard Methods
Other
E. coli – EC + MUG
Fecal Coliform – MPN
Heterotrophic Bacteria
Total Coliform - MPN
DHEC 2802 (8/2012)
EPA 1104
SM 9222 G (19th)
SM 9221 E (18th)
SM 9215 B
SM 9221 B
Page 27 of 33
R. Quality Control: Check the quality control practices that apply to your laboratory with the frequency performed, or list as
N/A if not applicable to your laboratory operations.
Quality Control
Yes
No
Frequency
Comments
29
Quality Assurance Plan
Standard Operating Procedures29
Initial Demonstration of Precision and Accuracy for
each Method29
Method Detection Limit Study29
Chain of Custody29
Sample Identification System
Use of Unknown PT samples29
Documented Standard Curve for each Method and
Analyte
Standard Curve Checked Prior to each Sample Set
Verify Curve Every Ten Samples
Laboratory Reagent Water Blanks
Use of Spiked Samples for Recovery Data
Use of Known Reference Samples
Use of Duplicate Samples
QC Charts or Tabulations
Service Contract on Balances
Use of ASTM Class 1, 2, or 3 Weights
Dating of Chemicals (received and opened)
Chemical Inventory Log
Standard Preparation Records
Column Inventory Log
GC, GC/MS Maintenance Log
Use of Field and/or Trip Blanks
Use of Field Duplicates
Use of Laboratory Control Samples
DHEC 2802 (8/2012)
Page 28 of 33
S. Statement of Validation
I have read South Carolina State Regulation 61-81, titled State Environmental Laboratory Certification. In accordance
with that Regulation, as the designated Laboratory Director, I submit this completed Application to the State
Environmental Laboratory Certification Program. I attest that the information on pages 1-28 is true, accurate and
complete to the best of my knowledge. I agree to notify the State Environmental Laboratory Certification Program within
15 days of changes in laboratory name, ownership, laboratory director, location, personnel, facilities, equipment,
methodology, and/or record keeping practices, or any other factors which might impair the ability of the laboratory to
perform in accordance with the terms of certification documented in Regulation 61-81.
With the attached application(s), I hereby apply for certification in accordance with the terms listed in South Carolina
Environmental Laboratory Certification Regulation 61-81.
_____________________________________
Name of Laboratory Director (type or print)
__________________________
Signature of Laboratory Director
DHEC 2802 (8/2012)
____________
Date
Page 29 of 33
T. Designation of Laboratory Director
THIS IS A SAMPLE FORM.
YOUR LABORATORY’S LETTERHEAD
Director, Office of Environmental Laboratory Certification
S.C. Department of Health and Environmental Control
2600 Bull St.
Columbia, South Carolina 29201
Re: Laboratory ID#______________ (If Laboratory is currently certified)
Dear Director:
In accordance with South Carolina State Environmental Laboratory Certification Regulation 61-81, Section D(12), as
proprietor of
, I designate
as the
Laboratory Director. He/she has the responsibility of supervising the operations of the laboratory and insuring the quality
and accuracy of the data reported. If there is a change in the Laboratory Director, I agree to notify the Office of
Environmental Laboratory Certification within 15 days of this change.
______________________________________
(Proprietor's Signature and Date)
______________________________________
(Type or Print Name)
_____________________________________
(Type or Print Title)
DHEC 2802 (8/2012)
Page 30 of 33
Application Footnotes
1
Safe Drinking Water Act:
For the approved methodology for Safe Drinking Water Act compliance, refer to 40 CFR Parts 141.21, 141.23,
141.24, 141.25, 141.74, 141.131, 141.402, 141.403, 143.4 and Appendix A to Subpart C of Part 141.
Clean Water Act:
For the approved methodology for Clean Water Act compliance, refer to 40 CFR Part 136, “Guidelines Establishing
Test Procedures for the Analysis of Pollutants under the Clean Water Act.”
Also refer to 40 CFR Part 403, “General Pretreatment Regulations for Existing and New Sources for Pollution,” 40
CFR Part 423, “Steam Electric Power Generating Point Source Category”, 40 CFR Part 430, “The Pulp, Paper, and
Paperboard Point Source Category,” 40 CFR Part 439, “Pharmaceutical Manufacturing Point Source category,” 40
CFR Part 455, “Pesticide Chemicals,” 40 CFR Part 465, “Coil Coating Point Source Category,” and 40 CFR Part 503,
“Standards for the Use or Disposal of Sewage Sludge.”
Solid and Hazardous Waste Testing:
For Solid and Hazardous Waste testing, the EPA approved method reference is SW-846, Third Edition of “Test
Methods for Evaluating Solid Waste, Physical/Chemical Methods”, amended by Updates I, II, II, IIA, IIB, III, IIIA, IIIB
IVA and IVB.
Also refer to 40 CFR Parts 260, 261,264, 265, 268, and 270, “Hazardous Waste Management System; Testing and
Monitoring Activities”.
2
MI agar also may be used. Preparation and use of MI agar is set forth in the article “New Medium for the
Simultaneous Detection of Total Coliform and Escherichia coli in Water” by Brenner, K.P.,et. al., 1993, Appl. Environ.
Microbiol. 59:3534-3544.
3
Colilert is also known as the ONPG-MUG test.
4
A description of the Colisure Test Feb. 28, 1994 can be obtained from IDEXX Laboratories, Inc. One IDEXX Drive,
Westbrook, Maine 04092. Phone: 800-321-0207. The website is www.idexx.com.
5
Reserved
6
A description of the m-ColiBlue24® Test, Aug.17, 1999 is available from the Hach Company.
7
Reserved
8
This method is not approved for compliance samples for the disinfection by-product rule.
9
National Council for the Paper Industry for Air and Stream Improvement, Technical Bulletin 253, December 1971.
10
Reserved
11
Under the disinfection by-product rule, the amperometric titration or spectrophotometry may be used for routine daily
monitoring of chlorite at the entrance to the distribution system, as prescribed in §141.132(b)(2)(i)(A). Ion
chromatography must be used for routine monthly monitoring of chlorite in the distribution system as prescribed in
§141.132(b)(2)(i)(B) and (b)(2)(ii).
12-13 Reserved
14
PCBs are quantitatively identified as Arochlors and measured for compliance purposes as decachlorobiphenyl.
15
Method detection limit studies must be submitted for all Arochlors (PCBs).
DHEC 2802 (8/2012)
Page 31 of 33
Application Footnotes (Cont.)
16
Users of Method 505 may have more difficulty in achieving the required detection limits than users of Methods 508.1,
525.2 or 508.
17
Enterolert, IDEXX Laboratories, Inc.
18-23 Reserved
24
Recommended for enumeration of target organism in sewage sludge.
25
Must be accompanied with the applicable metals and organic method certification.
26
Must be accompanied with the applicable volatiles certification.
27
Must be accompanied with the distillation procedure.
28
The extraction or sample preparation method needed will be based on the matrix and analytes of interest.
29
Must accompany the completed application form.
30
EPA Method 1624C and 1625C are for use with pharmaceutical effluents.
31
Distillation and analysis by an approved total cyanide method is required following the chlorination and dechlorination
treatments.
32
Available cyanide, Method OIA-1677 (Available Cyanide by Flow Injection, Ligand Exchange, and Amperometry),
ALPKEM, A Division of OI Analytical, PO Box 9010 College Station, TX 77842-9010. The website is
www.oianalytical.com.
33
The description for the Kelada 01 Method, “Kelada Automated Test Methods for Total Cyanide, Acid Dissociable
Cyanide, and Thiocyanate”, revision 1.2, August 2001, EPA# 821-B-01-009 for cyanide is available from the National
Technical Information Service (NTIS), PB 2001-108275, 5285 Port Royal Road, Springfield, VA 22161. The toll free
telephone number is 800-553-6847. The website is www.ntis.gov.
34
The description for the QuickChem Method 10-204-00-1-X “Digestion and Distillation of Total Cyanide in Drinking
and Wastewaters Using MICRO DIST and Determination of Cyanide by Flow Injection Analysis”, Revision 2.1,
November 30, 2000 for cyanide is available from Lachat Instruments, 6645 W. Mill Rd., Milwaukee, WI 53218, USA.
Phone: 414-358-4200. The website is www.lachatinstruments.com.
35
Reserved
36
A description of the SimPlate method, “IDEXX SimPlate TM HPC Test Method for Heterotrophs in Water”, November
2000 can be obtained from IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092. Phone: 800321-0207. The website is www.idexx.com.
37
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below
10oC during transit.
38-39 Reserved
40
Colitag® product for the determination of the presence/absence of total coliforms and E.coli is described in ``Colitag ®
Product as a Test for Detection and Identification of Coliforms and E.coli Bacteria in Drinking Water and Source
Water as Required in National Primary Drinking Water Regulations,'' August 2001, available from CPI International,
Inc., 5580 Skylane Blvd., Santa Rosa, CA, 95403. Phone (800) 878-7654. The website is www.cpiinternational.com.
DHEC 2802 (8/2012)
Page 32 of 33
Application Footnotes (Cont.)
41
In Vitro Determination of Chlorophyll a and Pheophytin a in Marine and Freshwater Algae by Fluorescence,"
Revision 1.2, September 1997. Nation Exposure Research Laboratory, Office of Research and Development, U.S.
Environmental Protection Agency, Cincinnati, Ohio 45268.
42
Sample preparation for fecal coliform analysis in biosolids samples is addressed in the EPA publication
“Environmental Regulations and Technology Control of Pathogens and Vector Attraction in Sewage Sludge” EPA
document EPA/625/R-92/013. The current version of this document is July 2003. Appendix F addresses the proper
techniques and dilutions for preparing biosolids samples for analysis of fecal coliforms using membrane filtration or
the Most Probable Number analytical techniques. This method not required if using EPA Methods 1680 or 1681.
43
The Gamma Emitters category includes Barium 133, Cesium 134, Cesium 137, Cobalt 60, and Zinc 65.
44
This method is not approved under 40CFR Part 136. It is approved only for Part 503 biosolids.
45
Ion chromatography & post column reaction or IC/ICP-MS must be used for monitoring of bromate for purposes of
demonstrating eligibility of reduced monitoring, as prescribed in §141.132(b)(3)(ii).
46
Samples must be preserved at the time of sampling with 50mg ethylenediamine (EDA)/L of sample and must be
analyzed within 28 days.
47
Inorganic carbon must be removed from the samples prior to analysis. TOC samples must not be filtered prior to
analysis. TOC samples must be acidified at the time of sample collection to achieve pH less than or equal to 2 with
minimal addition of the acid specified in the method or by the instrument manufacturer. Acidified TOC samples must
be analyzed within 28 days.
48
DOC samples must be filtered through 0.45-µm pore-diameter filter as soon as practical after sampling, not to
exceed 48 hours. After filtration, DOC samples must be acidified to a pH of less than or equal to 2 with minimal
addition of acid specified in the method or by the instrument manufacturer. Acidified DOC samples must be analyzed
within 28 days of sample collection. Inorganic carbon must be removed from the samples prior to analysis. Water
passed through the filter prior to filtration of the sample must serve as the filtered blank. This filtered blank must be
analyzed using procedures identical to those used for analysis of the samples and must meet the following criteria:
DOC
