Standards for Internal Form

Instructions and help about Standards For Internal

ok ladies and gentlemen it is time to start talking about internal standards internal standards can be a little bit of a challenge to figure out a purse but they're really not that bad after all so the reason why we do this is if we have some sort of loss of solution or perhaps some sort of reproducibility error with our injections and what you need to have is you have some sort of analyte which we'll call a and a standard that you call s and we want to set up a proportion between these two so ultimately our goal is to work with the equation that we have some response some areas for analyte and for standard and that those things depend on concentration so they're proportional to the concentration of the analyte or the standard and that there's just a proportion some sort of factor between the two of these so let's say that we mix known amounts of a and s and it turns out that we've got 20 milli molar sub a and 10 millimolar s and then you're going to measure them and let's just say lik