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Fill and Sign the Bir Form 1904 Editable

Fill and Sign the Bir Form 1904 Editable

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BABEC PCR Optimization Debriefing Form 1. Teacher Information Name_________________ School________________ 2. Partnership_________________________ 3. Subject/ Class (select all that apply) Adv./ Honors Biology AP Biology Biology Biotechnology Cell Biology Chemistry Environmental Science Forensics Genetics Integrated Science Other, please specify _____________________ 4. Provide the number of students for each section. If you marked multiple classes, above, please specify the class on each line (ex. Adv. Bio – 30, Biology – 32): Section 1______________ Section 4______________ Section 2______________ Section 5______________ Section 3______________ 5. What previous micropipetting experience did your students have? 6. Did you have major problems with your PCR reaction (ie. no bands)? Yes No 7. If you marked yes, please describe the reagent storage conditions. Check the appropriate box for each reagent. Reagents Fridge Freezer Room Temp. N/A 10X PCR Buffer 25mM MgCl2 10mM dNTP mix AmpliTaq Gold AChE forward primer AChE reverse primer Calf thymus DNA MW Marker Return forms to: BABEC Outreach Coordinator, c/o SCCBEP, San José State University, Department of Chemistry, San José, CA 95192-0101. 8. Tell us about the results of the teams optimizing the MAGNESIUM concentrations: # of teams ______ # of teams with bands ______ # of teams able to find an optimal concentration ______ # of teams with no results ______ 9. Were the bands distinct and easily readable? Yes No Comments: 10. Tell us about the results of the teams optimizing the PRIMER concentrations: # of teams ______ # of teams with bands ______ # of teams able to find an optimal concentration ______ # of teams with no results ______ 11. Were the bands distinct and easily readable? Yes No Comments: 12. Was a ladder run on all of the gels? Yes No Comments: 13. Did you see bands other than at 553 bp? Yes No Comments: 14. If your results did not turn out as expected, what problem(s) do you think may have occurred? 15. How was the support you received? Please specify the source (another teacher, partnership coordinator, BABEC outreach coordinator, etc.). 16. What additional support would have been helpful? 17. What was your students' overall reaction to the lab/unit? If possible, please include a quote or anecdote. 18. Additional comments: Return forms to: BABEC Outreach Coordinator, c/o SCCBEP, San José State University, Department of Chemistry, San José, CA 95192-0101.

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