Streamline Your Paperwork with the Hardware Bill Format for Life Sciences

Hardware bill format for Life Sciences

foreign welcome to quantalife I'm Bill Colston and I'm the CEO I'm here to talk to you about the team and the technology that's developing one of the first DNA Diagnostics for viruses such as the H1N1 flu we started developing this technology five years ago at one of the nation's Premier Research Laboratories at the time we were trying to rapidly detect bacteria and viruses it took hours to days to make this measurement here at quantalife reducing that down to minutes for the last 20 years Lawrence Livermore National Laboratory has built the country's largest biological defense program we established the science and reduces technology down to practice now the team and the technology have come together to form quantilife to commercialize this product and bring it to Market we are quantalife and here's how we do it my name is Isaac bright and I'm vice president of molecular Diagnostics here at quantolife my goal is to align our strategy around developing molecular diagnostic kits that provide very high clinical utility we have proprietary oil chemistries proprietary thermal cycling systems and detection systems that when working in concert allow us to provide nucleic acid testing on a scale that's never been done in a time frame that's never been seen before currently when most systems are operating with a central lab surveillance system it takes at a minimum six to eight hours if they're providing polymerase Chain Reaction testing oftentimes two days if they're working with a culture system with our system you're talking about having definitive results on the order of 10 to 15 minutes so there are many tests on the market now for agents such as methyl and resistant staph aureus or Vancomycin resistant enterococcus and our main goal in competing in those particular spaces would be to bring those tests to the point of care so that you had meaningful results from the test from that particular patient in the time that it took to triage the patient the system will be a very easy system to use your typical nurse or technician should be able to operate it at or near the point of care when you think about the need to respond to infectious outbreaks you need to be able to have definitive data about a patient's status so you can put them in the right place and start immediate therapy appropriately the course of their infection will then be much less you've decreased exposure to other patients that are adjacent you've decreased exposure to healthcare workers because in total the the course of their infection is much shorter my name is Nick Heredia and I'm in charge of assay development my role here is to streamline the process of developing new test kits a new test kit can easily be integrated into our platform by simply changing out the cartridge and this is very analogous to the way an inkjet printer cartridge fits into a printer the facility we're in right now here at Quant life is a biocontainment facility so we're capable of developing new assays for a wide variety of viral or bacterial pathogens we can develop a new test kit in about two months and if there's already an assay out there we can rapidly adapt it to our system so my name is Amy Henderson and my role is to ensure that the internal chemistry is reliable and robust the idea of the system is to make faster smaller reactions using micro droplet technology the micro droplet is a mini reactor that contains a single copy of DNA which can be from a virus or bacterium we know how much material we're starting with in the reaction therefore the analysis is quantitative droplets are made in an inexpensive disposable cartridge inside this cartridge we're able to make droplets that all have the same or uniform size we have a internal Quality Control process whereby we watch the stability of the droplets through the device we're looking for any changes or instabilities in these droplet reactors as they go through each step in the reaction each droplet can be thought of as an indestructible test tube and the advantage of making individual droplet reactors is so that we eliminate the possibility of cross-contamination my name is Don mascalaire and I'm a senior design engineer here at quantum my role is to take basic concepts that people have come up with and to gear towards the final product our concept of a thermal cycler is is much different than conventional PCR and conventional PCR you're you have to take a fairly large volume which which adds up to high thermal mass and you need to raise and lower the temperature of that volume which requires a fair amount of time in our system because we have Micro droplets we can change the temperatures very rapidly so what we do is we take a small curved pieces of metal and on the back side we actually put a heater and we make up a segmented device and then the capillary tube is simply wound around the outside of the metal as the droplet is transitioning from one region to the next probably within a second it's to the proper temperature so there's very little time wasted in transitioning from one temperature to the next so the technique of doing multiple tests in the quanticore is quite simple and once you've moved the first batch of sample through at that point you can add a slug of oil that becomes the separator from the next sample there's very few parts and very few moving parts my name is Kevin Ness and I am a initial founder I'm currently the director of research and development what I do here is basically make sure that every droplet counts the droplets after being thermally cycled for a few minutes are put back in single file and pass through the detection site the droplets move past a laser beam and as you can see now each flash of light is analyzing a single droplet on the scope each one of these spikes is information of what's occurring within the droplet the large spikes correspond to positive droplets that initially started with DNA the smaller spikes are droplets that did not start with DNA so once they're monitored I take the ratio of pauses and negatives and that gives us a concrete answer of exactly the starting sample concentration my job is to make sure that the results are accurate and reported in simple actionable terms for the clinician my name is Ben Hansen I'm the chief science officer here at quantolife my role is to oversee the scientific development of our diagnostic pipeline so develop our kits and our instrument and get it in the hands of our customer as quickly as possible the instrument is designed to be a point of care type instrument so it's the size of an inkjet printer runs off standard power and can generate lab quality results in a matter of 15 minutes so tests that typically take days to run you can get those results almost instantaneously the device is very simple to use all you have to do is take a swab from the patient break that swab off into our disposable cartridge and then essentially Downstream from that the instrument does the rest so it takes the bacterial virus breaks it open releases nucleic acid washes it and then concentrates it into a very small volume that volume then is converted into droplets within the cartridge those droplets then flow very quickly through a capillary tube in that capillary they're heated and cooled very rapidly which enables billions of copies of DNA to be made after amplification the droplets flow single file through a fluorescence detector that's exciting it with a laser beam and it's measuring how bright each droplet is and then that gives us the amount of DNA that was present in the sample and tells us whether or not in fact that harmful bacteria or harmful virus was present in that patient's specimen from a simple swab test we can get laboratory quality results in less than 15 minutes that's what this technology is all about we are going to transform the way doctors and clinicians treat patients foreign foreign