Fax Acceptor Initials with airSlate SignNow

Fax acceptor initials

so our next speaker is unwelcome Jenko he just told me that he even updated his presentation last minute before he came here so so probably you had some good ideas last night which you wanted to include it so this presentation imaging integrity of lipid nano carriers in bloodstream and tumour of living nice so we are looking forward to your last slide from you wanna yeah thank you chairman and thank you the organizers yep so okay I can see like this okay the other in our lab actually we are working on different type of particles and oops ah blah okay yeah different type of particles starting from very small ones which are brought inside my flow particles and then died of polymer nano particles this a little bit larger 10 to 80 nanometers and then even larger objects are lipid particles and the applications are very different like soup resolution for the small ones and fluorescence imaging and then cell tracking and detection of target biomolecules for polymer systems and lipid based particles are particularly suitable for in vivo imaging so here there I will focus on this part and why lipid nanoparticles and what is that so those in our case those are non emulsions essentially which are composed of the oil core and this hairy regulated surfactant and the important thing that the components are generally recognized as safe grass materials and the kulluk for or crema for which is a surfactant it's it is FDA approved injectable material and it's actually a component of pocket XL chemotherapy so but the thing is so instead of drugs you can put drugs inside or contrast agents so here I will focus on contrast agents fluorescent markers which aim which will reveal some interesting things about this particle so how they are made it's a very simple protocol this is another advantage of this system that you actually makes oil and oil surfactants you hit that at 40 degrees and then after mixing you you add water and spontaneous emulsification produce particles and the size of these particles depends actually on the ratio of oil and surfactant so it's that simple and you can go from 20 nanometers up to to almost 200 nanometers but the thing is that when one article when we publish the reviewer said these are not particles these are just droplets and okay it's a definition but it's true that these are liquid systems how well they encapsulate their the carbon molecules and because their core is liquid and our earlier studies we first naively tried now red which is a kind of model drug molecule which is also a lipophilic and it was binding well the core but once you inject for example in the zebrafish you see a very nice leakage of this dye everywhere in the tissue and later on we show that actually by using highly hydrophobic molecules like sign in and this bulky hydrophobic entrain which makes a unique molecules very up order we can first load much more dye inside like 40 times more and you can see this is the image of zebrafish you could you could see a blood circulation exclusively you don't see any any leakage so it's very important the hydrophobicity of their of the system or of the of the cargo to preserve inside droplet so now the key question was how stable are those droplets in vivo because they are essentially liquid systems so and if we apply them for for drug delivery they are supposed to bring the cargo molecule up to the tumor and so we wanted to follow all the way up to two more whether they Carver will be inside and for that the unique approach is so-called fret first the resonance energy transfer where we can put two fluorescent dyes together when they're together we will see the acceptor initial and when they get decomposed they will see only donor mission because donor and acceptor molecules get separated so but for that we need dyes which operate between 700 and 800 nanometer which is an optical window where the tissues are transparent so you can use a microscope fluorescence microscope where we can face a whole animal imaging so our system was it was a couple of new infrared dyes sign in design in five point five and seven point five with long alkyl chains so again very hydrophobic and we needed this magic on trying to make those guys well soluble because their ionic and so we need a control which is very hydrophobic and so like this we replace these guys together in the droplet at high concentration to produce this is this energy transform and so this is their first we have to optimize the formulation so we change the concentration of our fret pair and when we get up to 1% so we observe decrease of the donor emission and increase of the acceptor emission which is normal because the at high concentration donor acceptor gets closer to each other and you have nice fret so so 1% was chosen as the working concentration then we have to check whether our system works when we make a disruption of the particle by the organic solvent we could dioxin it it we we observe that initially fret system with the acceptor relation turns into exclusively donor nation because donor acceptor separates so the system works and now first test we did stability in serum so when you add 100% serum you you have only little change in the ratio of the acceptor versus donor or versus the sum of acceptor donors it doesn't matter so the changes are small and of course in in water there was no change so it is stable in serum so the next thing when we want to quantify the integrity of the system we have to calibrate first our device and so our imaging setup and for that also we have to have a model of the integrity and for that so we mixed actually fully the 100% integrity particle which is our 1% red pair and separately donor and acceptor as supposed to be 0% so we mix them and then we check the spectrum as expected the donor increases when integrity decreases with respect to exception we mix these guys and put them in the vase and do imaging so this is a donor which is increasing when integrity decreases and acceptor slightly decreases then we make a ratio and this color actually reflects the integrity of the system so once we have have that we perform the calibration where this ratio of the channels reflects the integrity so then we injected into mice and what we could see actually that we have a strong acceptor emission which reflects their threat in our mice and especially in the blood circulation you can see in the tail vein and you can see also in the liver so then this color actually is mapped for the integrity which you can be called from from these colors actually each color and here the color red means hundred percent integrity in the blood circulation and the rest is already less so with time you can see that the donor fluorescence increases and accept a fluorescent decreases so what happened actually that the particles slowly did disintegrate and when you place inspect in the region of interest like like liver you can see there is a disintegration with a half-life of 8.2 hours and for instance in the blood circulation which we focused actually on there belvane there was almost no change so those nano droplets are very stable in the blood circulation but in liver date they decompose the same we did with the cleaner grafted tumor so it's a memory considered carcinoma and what was nice that oops that part these particles accumulated rapidly within one hour in the tumor and then important that the fret signal was very high which which actually reflected that we have almost 100% integrity once the droplets get into the tumor so this was really important to see that those liquid system can enter tumor in nearly intact form but with time we observe the disintegration of the system with the half-life of about four four point four hours and of course we check whether it after dissecting the knife we could see that it selectively gets into two more more than into liver so in conclusion try to present you is there system based on lipids those lipids another droplet can be made of any size that we can load them with organic molecules especially with this fluorescent dyes and quite efficiently effectively and then these are components of this system are FDA approved and we describe the method of imaging based on fret which will allow you to quantify the integrity of of the nano system and in this case the lipid so importantly those lipid systems can accumulate into tumor guess this is by EPR because we did not have any targeting ligand and they importantly they disintegrate only after entering the target tumor so which is important for future application in in drug delivery and so the next natural step is to go for drug delivery with these systems and so I would like to thank my group which works hard on different field but it's those projects or carried out mainly by red one which Allah who is present here and his poster number eight is here and then Bogdan hundred synthesize the molecules then there so they've biophotonics groups who assist us with some help results with instrumentation imaging and the collaborators especially Jacky Goods and Nicole Anton and Van Damme and from Strasbourg and of course I have to take the financial support especially ERC and the others so thank you for for your attention thank you very much for your a nice presentation this morning must be a lot of questions to you circulation time of view particles in blood yeah the circulation time we measured from directly from fluorescence and through fluorescence intensity we could have about three hours cos life circulation but insect it is longer I think we are limited by their signal I think we have to directly take the blood samples and check where because we see key scan experiments we get 8 hours half-life circulation but I yeah it's a fluorescent images could be very misleading actually the particles could be deposited in the skin for example in just imaging the skin deposition instead of through blood-borne particles ah yeah well here here actually the in fact we are we are quite happy with the quality of those image oops with the quality of those images because you you clearly see actually localization in the tail rail and this signal disappears actually within within 6 hours you don't see anything it can be seen here you have here a clear signal there the signal actually doesn't change much in the whole body and then it disappears in the tail vein so that's not skin and when you see skin is just a homogeneous for us on the surface that we can distinguish is really on the image but of course we have to be careful with interpretation Thank You Andre for a very nice presentation so how readily is this system transferable for instance to liposomes or other nano carriers where you can use the frick principle in order to you know see this integration as a principle yeah I think the key difference in the core because in life was from the quarries is water here the core is oil so therefore in principle the difference that we can solubilize lipophilic material directly inside the core and for the threat experiments the threat experienced in vesicles is done mainly on the membrane or if you can encapsulate then you have to precipitate something inside the liposome but but I would say the quantification of integrity with the leak liquid system liquid core oil core is very straightforward because you can calculate precisely how much we have inside and how much has been released here because it's a simple solution of organic dioxide oil yes I don't know if I yes you can use the membrane of liposome but the problem when you use dyes at the membrane surface there are too much accessible to the proteins of serum and you will have a leakage very fast it's extremely fast we observe this with membrane probes they its minute but when it's hidden in the core its hours or more yeah anybody else if I understood it correctly yessing in the second slide you said that your droplets call it that way up pegylated yes so it's a dear the surface is actually covered by Kaali for which is there which is a one side is a lipophilic part and you have a peg of about a thousand molecular weight so we have a it's a Val pegylated channel yeah I'm just asking the question tomorrow there will be a presentation on pickle and that a lot of crap our reactions have been seen if let's say nano particles or droplets liposomes are regulated have you looked into that issue already in their trouble reactions kappa reaction that means let's say you get a type of let's say IgG reactions Title IVD way and in fact the califor is known to have allergic effects but ok it is still at the approved material compared to many other surfactants which I even more toxic but we did not check that I think once we get into the application real application therefore the imaging stage the doses are actually very low and then when we have to go to the delivery we have to increase like 20 times with those so then I think all problems may start yeah I just came to this point because I know that the FDA just is looking into that and have some new regulations ok we all suffer form I would say if you get to the more let's say practical clinical point ok ok thank you very much [Applause]