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Integrate multiplex calculated

this video covers working with multiplex assays and how data analysis is typically performed multiplex assays allow you to measure results for multiple analytes with a single test these are bead-based assays with multiple binding regions on each bead analyte specific capture antibodies are bound to specific regions which are identified with a red laser and quantified using a green laser the advantages of using multiplex assays include requiring less sample volume reagent and time to return results for multiple analytes common manufacturers of multiplex assays include luminex and msd although there are many benefits to running multiplex assays there are also challenges if you're running a multiplex assay with a large number of analytes for example 500 different analytes this creates a large amount of data organizing the data to be imported into an analysis software can be difficult if the software is not optimized to accept your measurement data file additionally performing curve fitting for multiple analytes can be time consuming if performed individually or will require a data analysis software powerful enough to perform multiple fits at once there are three main ways multiplex assays are typically analyzed quantitative assays perform a curve fit for each analyte and concentration results are interpolated from the curve semi-quantitative assays perform a curve fit for each analyte but those concentration results are reported as qualitative designations such as positive or negative based on threshold values qualitative assays do not require a curve fit to be performed for each analyte the qualitative results are reported based on threshold values or by comparing the results to other samples on the run such as control samples myassays offers several tools for analyzing multiplex data the first of which is my assays desktop a full laboratory data analysis solution including compatibility with major multiplex assay manufacturers so measurement data files can be automatically imported with no modification or formatting required the powerful curve fitting tools of myassay's desktop allow you to apply advanced curve fitting and weighting to all analytes the fit method can be specified for all analytes each analyte individually or using the best fit feature to automatically compare and apply the best fit method as determined by the specified goodness measure the reports created are fully customizable and easily exported to excel word as a pdf or a custom export for limbs this example demonstrates the curve and sample table reported for an analyte the tabs sections at the bottom of the screen show the results for each analyte this example shows a qualitative assay with results for each analyte contained in a single sample table reporting positive or negative designations the next data analysis tool is the my curfit excel add-in this option allows you to complete the analysis from directly within excel using complex curve fit methods like 4pl and 5pl the mycurfet xl add-in is a cost-effective tool appropriate for users with the knowledge of excel once the spreadsheet has been successfully configured data can be copied and...