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[Music] there's actually a major health crisis today in terms of the shortage of organs the fact is that we're living longer Medicine has done a much better job making us live longer and the problem is as we age our organs tend to fail more and so currently there not enough organs to go around in fact in the last ten years a number of patients require an organ has doubled well in the same time the actual number of transplants has barely gone up this is now a public health crisis so that's where this field comes in that we call the field of regenerative medicine it really involves many different areas you can use actually scaffolds biomaterials they're like the piece of your blouse or your shirt but specific materials you can actually implanted patients and they will do well and help you regenerate or we can use cells alone either your very own cells or different stem cell populations or we can use both we can use actually biomaterials and the cells together and that's where the field is today but it's actually not a new field interestingly this is a book that was published back in 1938 titled a culture of organs the first author Alexis Carrel and Nobel Prize winner he actually devised some of the same technologies used today for suturing blood vessels and some of the blood vessel grafts were used today were actually designed by Alexis but I want you to note his co-author Charles Lindbergh that's the same Charles Lindbergh who actually spent the rest of his life working with Alexis at the Rockefeller Institute in New York in the area of the culture of organs so the fields been around for so long why so field clinical advances and that really has to do to many different challenges but if I were to point to three challenges the first one is actually the design of materials that could go in your body and do well over time and many advances now we can do that fairly readily the second challenge with cells we could not get enough of your cells to grow outside of your body over the last 20 years years we've basically tackled that many scientists can now grow many different types of cells plus we have stem cells but even now 2011 there's still certain cells that we just can grow from the patient liver cells nerve cells pancreatic cells still can grow them even today and the third challenge is vascularity the actual supply of blood to allow those organs or tissues to survive once we regenerate them so we can actually use biomaterials now this is actually biomaterial we can weave them knit them or we can make them like you see here this is actually like a cotton candy machine you saw this spray going in that was like the fibers of the cotton candy creating this structure this tubular eye structure which is a biomaterial that we can then use to help your body regenerate using your very own cells to do so and that's exactly what we did here because it's actually a patient who presented with a deceased organ and we then created one of these smart biomaterials and we then use that smart bomb material to replace and repair that patients structure what we did was we actually use the biomaterial as a bridge so that the cells in the organ could walk on that bridge if you will and help to bridge the gap to regenerate that tissue and you see that patient now six months after with an x-ray showing you the regenerated tissue which is fully regenerated when you analyze it under the microscope we can also use cells alone these are actually cells that we obtained these are stem cells that we create from specific sources and these are we can drive them to chem heart cells and they start beating in culture so they know what to do the cells genetically know what to then they start beating together now today many clinical trials using different kinds of stem cells for heart disease so that's actually now in patients or if we're going to use larger structures to replace larger structures we can then use the patient's own cells or some cell population and the biomaterials the scaffolds together so the concept here is if you do have a or injured organ we take a very small piece of that tissue listen half the size of a postage stamp we then tease the cells apart we grow the cells outside the body we then take a scaffold a biomaterial again looks very much like a piece of your blouse or your shirt we then shape that material and we then use those cells to code that material one layer at a time very much like baking a layer cake if you will we then place that in an oven-like device and we're able to create that structure and bring it out this is actually a hard valve that we have engineered and you can see here we have the structure of the heart valve and we've seated that with cells and then we exercise that you so you see the leaflets opening and closing of this heart valve that's currently being used experimentally to try to get it to to further studies another technology we have used in patients actually involves bladders we actually take a very small piece of a bladder from the patient less than half the size of a postage stamp we then grow the cells outside the body take the scaffold coat the scaffold with the cells the patient's own cells two different cell types we then put it in this oven like device has the same conditions as a human body 37 degrees centigrade 95% oxygen a few weeks later you have your engineered organ that we're then able to implant back into the patient for these specific patients we actually just suture these materials we use three-dimensional imaging analysis but we actually created these biomaterials by hand but we now have better ways to create these structures with the cells we use now some type of technologies where first solid organs for example like the liver what we do is we take discard livers as you know a lot of organs are actually discarded not used so we can take these liver structures which are not going to be used and we then put them in a washing machine like structure that will allow the cells to be washed away two weeks later you have something that looks like a liver you can hold it like a liver but it has no cells it's just a skeleton of the liver and we then can Reaper fuse the liver with cells preserving the blood vessel tree so we actually profuse first a blood vessel tree with the patient's own blood vessel cells and we then infiltrate the parenchyma with the liver cells and we not been able just to show the creation of human liver tissue just this past month using this technology another technology that we've used is actually that of printing this is actually a desktop inkjet printer but instead of using ink we're using cells and you can actually see here the printhead going through and printing this structure and it takes about 40 minutes to print this structure and there's a 3d elevator that then actually goes down one layer at a time each time the printhead goes through then finally you're able to get that structure out you can pop that structure out of the printer and implant it and this is actually a piece of bone that I'm going to show you in this slide that was actually created with this desktop printer and implant it as you see here that's all new bone that was implanted using these techniques another more advanced technology we're looking at right now our next generation of technologies are more sophisticated printers this particular printer with worthy signing now is actually 1 we print right on the patient so what you see here is I know it sounds funny but that's the way it works because in reality what you want to do is you actually want to have the patient on the bed with the wound and you have a scanner basically a cliff like a flatbed scanner that's what you see here on the right side you see a scanner technology that first scans the wound on the patient and then it comes back with the print hits actually printing the layers that you require on the patient's themselves this is how it actually works here's a scanner going through scanning the wound once it's scanned sends information in layers the correct layers of cells where they need to be and now you're going to see here a demo of is this actually being done in a representative wound and we actually do this with a gel so you can lift the gel material so once those cells are on the patient they will stick where they need to be and this is actually new technology still under development we're also working on more sophisticated printers because in reality our biggest challenge are the solid organs I don't know if you realize this but ninety percent of the patients and the transplant list are actually waiting for a kidney patients are dying every day because we don't have enough of those organs to go around so this is more challenging large organ vascular a lot of blood vessels supply a lot of cells present so the strategy here is this is actually a CT scan an x-ray and we go layer by layer using computerized morphometric imaging analysis and 3d reconstruction to get right down to those patient's own kidneys we then are able to actually image those do 360-degree rotation to actually analyze the kidney in its full volumetric characteristics and we then are able to actually take this information and then scan this in a printing computerized form so we go layer by layer through the organ analyzing each layer as we go through the organ and we then are able to send that information as you see here through the computer and actually design the organ for the patient this actually shows the actual printer and this actually shows that printing in fact we actually have the printer right here so I'm in while we've been talking today we've actually you can actually see the printer back here in the backstage that's actually the actual printer right now and that's been printing this kidney structure that you see here it takes about seven hours to print the kidneys this is about three hours into it now and dr. Kang's going to walk onstage right now and we're actually going to show you one of these kidneys that we print a little bit earlier today put a pair of gloves here thank you you're backwards so these clothes are a little bit small on me but here it is you can actually see that kidney as it was printed earlier today that's a little bit of consistency to it this is dr. Connie who's been working with us in this project and we went a part of our team Thank You dr. Kahn appreciate it so this is actually a new generation this is actually the printer that you see here on stage and this is actually a new technologies we're working on now in reality you know we now have a long history of doing this I'm going to share with you a clip in terms of technology that we have had in patients now for a while and this is actually a very brief clip only about 30 seconds of a patient who actually received an organ I was really sick I could barely get out of bed I was missing school it was just pretty much miserable I couldn't you know go out in play you know basketball at recess without feeling like I was going to pass out when I got back inside it was I felt so sick I was facing basically a lifetime of dialysis and I don't even like to think about what my life would be like if I was on that so after the surgery life got a lot better for me I was able to do more things I was able to wrestle in high school I became the captain the team and that was great I was able to be you know the normal kid with my friends and because they use my own cells to you know build this bladder it's gonna be with me I got it for life so I'm all set these experiments sometimes work and it's very cool when they do Luke come up please so look before last night when's the last time you saw Tony ten years ago when I had my surgery and it's really great to see him I'm tell us a little about what you're doing well right now I'm in college at the University of Connecticut I'm a sophomore and studying communications TV and mass media and basically trying to live life like a normal kid which I always wanted growing up but it was hard to do that when I was born with spina bifida and my kidneys and bladder weren't working I went through about 16 surgeries and it seemed impossible to do that when I was in kidney failure when I was ten and this surgery came along and basically made me who I am - and saved my life I'm Tony Dunne hundreds of these what I know from he's he's working really hard in his lab and coming up with crazy stuff I know I was one of first ten people to have this surgery and when I was ten I didn't realize how amazing it was I was a little kid and I was like yet I'll have that I'll have that surgery I all I wanted to do is to get better and I didn't realize how amazing it really was until now that I'm older and I see the amazing things that he's doing um when you know you got this call out of the blue I mean Tony's really shy and it took a lot of convincing to get somebody as modest as Tony to allow us to bring Luke so look you go to your communications professors your majoring communications and you ask them for permission to come to Ted which might have a little bit to do with communications and what was their reaction most of my professors were all for it and they said bring pictures and and you know show me the clips online and I'm happy for you there are a couple that were a little stubborn but I had to talk to I pulled him aside well it's an honor of privilege to meet you thank you so much you
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